Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients

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Abstract

Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays. Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. Results: Entirely concordant resistance levels were shown in 5/10 (50%) highly active antiretroviral therapy (HAART)-experienced patients, Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume51
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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Highly Active Antiretroviral Therapy
Protease Inhibitors
HIV-1
HIV
Viruses
Drug Resistance
Peptide Hydrolases
Therapeutics

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

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title = "Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients",
abstract = "Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays. Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. Results: Entirely concordant resistance levels were shown in 5/10 (50{\%}) highly active antiretroviral therapy (HAART)-experienced patients, Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.",
author = "Stefania Paolucci and Fausto Baldanti and Maurizio Zavattoni and Giuditta Comolli and Nazzarena Lab{\`o} and Stefano Menzo and Massimo Clementi and Giuseppe Gerna",
year = "2003",
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T1 - Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients

AU - Paolucci, Stefania

AU - Baldanti, Fausto

AU - Zavattoni, Maurizio

AU - Comolli, Giuditta

AU - Labò, Nazzarena

AU - Menzo, Stefano

AU - Clementi, Massimo

AU - Gerna, Giuseppe

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays. Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. Results: Entirely concordant resistance levels were shown in 5/10 (50%) highly active antiretroviral therapy (HAART)-experienced patients, Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.

AB - Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays. Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. Results: Entirely concordant resistance levels were shown in 5/10 (50%) highly active antiretroviral therapy (HAART)-experienced patients, Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.

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