TY - JOUR
T1 - Comparison of manual methods of extracting genomic DNA from dried blood spots collected on different cards
T2 - Implications for clinical practice
AU - Molteni, C. G.
AU - Terranova, L.
AU - Zampiero, A.
AU - Galeone, C.
AU - Principi, N.
AU - Esposito, Susanna
PY - 2013/7
Y1 - 2013/7
N2 - Isolating genomic DNA from blood samples is essential when studying the associations between genetic variants and susceptibility to a given clinical condition, or its severity. This study of three extraction techniques and two types of commercially available cards involved 219 children attending our outpatient pediatric clinic for follow-up laboratory tests after they had been hospitalised. An aliquot of venous blood was drawn into plastic tubes without additives and, after several inversions, 80 μL were put on circles of common paper cards and Whatman FTA-treated cards. Three extraction methods were compared: the Qiagen Investigator, Gensolve, and Masterpure. The best method in terms of final DNA yield was Masterpure, which led to a significantly higher yield regardless of the type of card (p
AB - Isolating genomic DNA from blood samples is essential when studying the associations between genetic variants and susceptibility to a given clinical condition, or its severity. This study of three extraction techniques and two types of commercially available cards involved 219 children attending our outpatient pediatric clinic for follow-up laboratory tests after they had been hospitalised. An aliquot of venous blood was drawn into plastic tubes without additives and, after several inversions, 80 μL were put on circles of common paper cards and Whatman FTA-treated cards. Three extraction methods were compared: the Qiagen Investigator, Gensolve, and Masterpure. The best method in terms of final DNA yield was Masterpure, which led to a significantly higher yield regardless of the type of card (p
KW - Dried blood spots
KW - Extraction
KW - Genomic DNA
UR - http://www.scopus.com/inward/record.url?scp=84887451614&partnerID=8YFLogxK
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M3 - Article
C2 - 24067477
AN - SCOPUS:84887451614
VL - 26
SP - 779
EP - 783
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
IS - 3
ER -