Comparison of manual methods of extracting genomic DNA from dried blood spots collected on different cards: Implications for clinical practice

C. G. Molteni, L. Terranova, A. Zampiero, C. Galeone, N. Principi, Susanna Esposito

Research output: Contribution to journalArticle

Abstract

Isolating genomic DNA from blood samples is essential when studying the associations between genetic variants and susceptibility to a given clinical condition, or its severity. This study of three extraction techniques and two types of commercially available cards involved 219 children attending our outpatient pediatric clinic for follow-up laboratory tests after they had been hospitalised. An aliquot of venous blood was drawn into plastic tubes without additives and, after several inversions, 80 μL were put on circles of common paper cards and Whatman FTA-treated cards. Three extraction methods were compared: the Qiagen Investigator, Gensolve, and Masterpure. The best method in terms of final DNA yield was Masterpure, which led to a significantly higher yield regardless of the type of card (p

Original languageEnglish
Pages (from-to)779-783
Number of pages5
JournalInternational Journal of Immunopathology and Pharmacology
Volume26
Issue number3
Publication statusPublished - Jul 2013

Keywords

  • Dried blood spots
  • Extraction
  • Genomic DNA

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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