Comparison of oncogenic HPV type-specific viral DNA load and E6/E7 mRNA detection in cervical samples: Results from a multicenter study

Francesco Broccolo, Lisa Fusetti, Sandra Rosini, Donatella Caraceni, Roberta Zappacosta, Lucia Ciccocioppo, Barbara Matteoli, Philippe Halfon, Mauro S. Malnati, Luca Ceccherini-Nelli

Research output: Contribution to journalArticle

Abstract

High-risk human papillomavirus (HR-HPV) genotype viral load and E6/E7 mRNA detection are proposed as surrogate markers of malignant cervical lesion progression. Currently, the use of commercially available DNA-based or mRNA-based tests is under investigation. In this study, the viral DNA load and E6/E7 mRNA detection of the five most common HR-HPV types detected in cervical cancer worldwide were compared in 308 cervical samples by using in-house type-specific quantitative real-time PCR assays and PreTect HPV-Proofer test, respectively. Sensitivity and negative predictive values were higher for the HPV-DNA assays combined (95.0% and 96.0%, respectively) than the RNA assays (77.0% and 88.0%, respectively); conversely, the mRNA test showed a higher specificity and higher positive predictive value (81.7% and 66.9%, respectively) than the DNA test (58.6% and 52.5%, respectively) for detecting histology-confirmed high-grade cervical intraepithelial neoplasia. A significantly higher association between viral DNA load and severity of disease was observed for HPV 16 and 31 (γ=0.62 and γ=0.40, respectively) than for the other HPV types screened. A good degree of association between the two assays was found for detection of HPV 16 (k=0.83), HPV 18 (k=0.72), HPV 33 (k=0.66), and HPV 45 (k=0.60) but not for HPV 31 (k=0.24). Sequence analysis in L1 and E6-LCR regions of HPV 31 genotypes showed a high level of intra-type variation. HR-HPV viral DNA load was significantly higher in E6/E7 mRNA positive than negative samples (P

Original languageEnglish
Pages (from-to)472-482
Number of pages11
JournalJournal of Medical Virology
Volume85
Issue number3
DOIs
Publication statusPublished - Mar 2013

Keywords

  • Cervical cytological samples
  • E6/E7 mRNA
  • High-risk HPV-genotypes
  • Viral load

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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