Comparison of pancreatic histology specimens obtained by EUS 19G versus 22G core biopsy needles: A prospective multicentre study among experienced pathologists

Maria Chiara Petrone, Jan Werner Poley, Matteo Bonzini, Ihab Abdulkader, Katharina Biermann, Genevieve Monges, Guido Rindi, Claudio Doglioni, Marco J. Bruno, Marc Giovannini, Julio Iglesias-Garcia, Alberto Larghi, Paolo Giorgio Arcidiacono

Research output: Contribution to journalArticle

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Abstract

Background and aim: Scanty data about inter-observer agreement (IOA) among pathologists in the evaluation of pancreatic samples acquired with EUS histology needle are available. The aim of this study was to determine IOA on adequacy of pancreatic histology specimens obtained with a 22G needle by a panel of experienced pathologist, in comparison with the 19G needle. Methods: This multicentre prospective study involved 73 pancreatic specimens prepared using histology needles of different calibres. Five pathologists independently reviewed all the samples, assessing the presence of a core, specimen adequacy and the possibility to perform additional analyses. IOA determined by Fleiss’ Kappa statistic was used as the primary outcome measure. Secondary outcome was to compare 22G versus 19G needle results. Results: A core was present in 57% of pancreatic specimens obtained by 22G needle. The specimens were considered adequate in 72% of cases, with poor agreement among pathologists (p = 0.02, Fleiss’ κ = 0.26). The possibility to perform further analyses was rated as ‘positive’ in 66% of cases without significant difference among observers (p = 0.80). When comparing the results, the presence of a core and the adequacy of tissue slides were significantly better for the 19G needle (57% vs. 84% p = 0.002; 72% vs. 83% p = 0.004, respectively). Reproducibility in the assessment of pancreatic sample adequacy was significantly better with the 19G needle (κ = 0.26 for 22G samples vs. κ = 0.81 for 19G samples). Conclusions: Our results suggest that histology sampling of pancreatic masses should be performed with a 19G histology needle, since is able to provide a core in the majority of cases, with 83% of adequate specimens and excellent results in term of reproducibility among pathologists.

Original languageEnglish
Pages (from-to)854-858
Number of pages5
JournalUnited European Gastroenterology Journal
Volume5
Issue number6
DOIs
Publication statusPublished - Oct 1 2017

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Large-Core Needle Biopsy
Multicenter Studies
Needles
Histology
Prospective Studies
Pathologists
Outcome Assessment (Health Care)

Keywords

  • Endoscopic ultrasound
  • experienced pathologists
  • histology needle
  • inter-observer agreement
  • pancreatic cancer

ASJC Scopus subject areas

  • Oncology
  • Gastroenterology

Cite this

Comparison of pancreatic histology specimens obtained by EUS 19G versus 22G core biopsy needles : A prospective multicentre study among experienced pathologists. / Petrone, Maria Chiara; Poley, Jan Werner; Bonzini, Matteo; Abdulkader, Ihab; Biermann, Katharina; Monges, Genevieve; Rindi, Guido; Doglioni, Claudio; Bruno, Marco J.; Giovannini, Marc; Iglesias-Garcia, Julio; Larghi, Alberto; Arcidiacono, Paolo Giorgio.

In: United European Gastroenterology Journal, Vol. 5, No. 6, 01.10.2017, p. 854-858.

Research output: Contribution to journalArticle

Petrone, Maria Chiara ; Poley, Jan Werner ; Bonzini, Matteo ; Abdulkader, Ihab ; Biermann, Katharina ; Monges, Genevieve ; Rindi, Guido ; Doglioni, Claudio ; Bruno, Marco J. ; Giovannini, Marc ; Iglesias-Garcia, Julio ; Larghi, Alberto ; Arcidiacono, Paolo Giorgio. / Comparison of pancreatic histology specimens obtained by EUS 19G versus 22G core biopsy needles : A prospective multicentre study among experienced pathologists. In: United European Gastroenterology Journal. 2017 ; Vol. 5, No. 6. pp. 854-858.
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abstract = "Background and aim: Scanty data about inter-observer agreement (IOA) among pathologists in the evaluation of pancreatic samples acquired with EUS histology needle are available. The aim of this study was to determine IOA on adequacy of pancreatic histology specimens obtained with a 22G needle by a panel of experienced pathologist, in comparison with the 19G needle. Methods: This multicentre prospective study involved 73 pancreatic specimens prepared using histology needles of different calibres. Five pathologists independently reviewed all the samples, assessing the presence of a core, specimen adequacy and the possibility to perform additional analyses. IOA determined by Fleiss’ Kappa statistic was used as the primary outcome measure. Secondary outcome was to compare 22G versus 19G needle results. Results: A core was present in 57{\%} of pancreatic specimens obtained by 22G needle. The specimens were considered adequate in 72{\%} of cases, with poor agreement among pathologists (p = 0.02, Fleiss’ κ = 0.26). The possibility to perform further analyses was rated as ‘positive’ in 66{\%} of cases without significant difference among observers (p = 0.80). When comparing the results, the presence of a core and the adequacy of tissue slides were significantly better for the 19G needle (57{\%} vs. 84{\%} p = 0.002; 72{\%} vs. 83{\%} p = 0.004, respectively). Reproducibility in the assessment of pancreatic sample adequacy was significantly better with the 19G needle (κ = 0.26 for 22G samples vs. κ = 0.81 for 19G samples). Conclusions: Our results suggest that histology sampling of pancreatic masses should be performed with a 19G histology needle, since is able to provide a core in the majority of cases, with 83{\%} of adequate specimens and excellent results in term of reproducibility among pathologists.",
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T1 - Comparison of pancreatic histology specimens obtained by EUS 19G versus 22G core biopsy needles

T2 - A prospective multicentre study among experienced pathologists

AU - Petrone, Maria Chiara

AU - Poley, Jan Werner

AU - Bonzini, Matteo

AU - Abdulkader, Ihab

AU - Biermann, Katharina

AU - Monges, Genevieve

AU - Rindi, Guido

AU - Doglioni, Claudio

AU - Bruno, Marco J.

AU - Giovannini, Marc

AU - Iglesias-Garcia, Julio

AU - Larghi, Alberto

AU - Arcidiacono, Paolo Giorgio

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background and aim: Scanty data about inter-observer agreement (IOA) among pathologists in the evaluation of pancreatic samples acquired with EUS histology needle are available. The aim of this study was to determine IOA on adequacy of pancreatic histology specimens obtained with a 22G needle by a panel of experienced pathologist, in comparison with the 19G needle. Methods: This multicentre prospective study involved 73 pancreatic specimens prepared using histology needles of different calibres. Five pathologists independently reviewed all the samples, assessing the presence of a core, specimen adequacy and the possibility to perform additional analyses. IOA determined by Fleiss’ Kappa statistic was used as the primary outcome measure. Secondary outcome was to compare 22G versus 19G needle results. Results: A core was present in 57% of pancreatic specimens obtained by 22G needle. The specimens were considered adequate in 72% of cases, with poor agreement among pathologists (p = 0.02, Fleiss’ κ = 0.26). The possibility to perform further analyses was rated as ‘positive’ in 66% of cases without significant difference among observers (p = 0.80). When comparing the results, the presence of a core and the adequacy of tissue slides were significantly better for the 19G needle (57% vs. 84% p = 0.002; 72% vs. 83% p = 0.004, respectively). Reproducibility in the assessment of pancreatic sample adequacy was significantly better with the 19G needle (κ = 0.26 for 22G samples vs. κ = 0.81 for 19G samples). Conclusions: Our results suggest that histology sampling of pancreatic masses should be performed with a 19G histology needle, since is able to provide a core in the majority of cases, with 83% of adequate specimens and excellent results in term of reproducibility among pathologists.

AB - Background and aim: Scanty data about inter-observer agreement (IOA) among pathologists in the evaluation of pancreatic samples acquired with EUS histology needle are available. The aim of this study was to determine IOA on adequacy of pancreatic histology specimens obtained with a 22G needle by a panel of experienced pathologist, in comparison with the 19G needle. Methods: This multicentre prospective study involved 73 pancreatic specimens prepared using histology needles of different calibres. Five pathologists independently reviewed all the samples, assessing the presence of a core, specimen adequacy and the possibility to perform additional analyses. IOA determined by Fleiss’ Kappa statistic was used as the primary outcome measure. Secondary outcome was to compare 22G versus 19G needle results. Results: A core was present in 57% of pancreatic specimens obtained by 22G needle. The specimens were considered adequate in 72% of cases, with poor agreement among pathologists (p = 0.02, Fleiss’ κ = 0.26). The possibility to perform further analyses was rated as ‘positive’ in 66% of cases without significant difference among observers (p = 0.80). When comparing the results, the presence of a core and the adequacy of tissue slides were significantly better for the 19G needle (57% vs. 84% p = 0.002; 72% vs. 83% p = 0.004, respectively). Reproducibility in the assessment of pancreatic sample adequacy was significantly better with the 19G needle (κ = 0.26 for 22G samples vs. κ = 0.81 for 19G samples). Conclusions: Our results suggest that histology sampling of pancreatic masses should be performed with a 19G histology needle, since is able to provide a core in the majority of cases, with 83% of adequate specimens and excellent results in term of reproducibility among pathologists.

KW - Endoscopic ultrasound

KW - experienced pathologists

KW - histology needle

KW - inter-observer agreement

KW - pancreatic cancer

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