Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems - Implications for use in bioartificial liver devices

M. T. Vilei, A. Granato, C. Ferraresso, D. Neri, P. Carraro, G. Gerunda, M. Muraca

Research output: Contribution to journalArticle

Abstract

The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed highest rates of diazepam metabolism (2.549±0.821 μg/h/million cells vs. 0.474±0.079 μg/h/million cells rats, p

Original languageEnglish
Pages (from-to)392-396
Number of pages5
JournalInternational Journal of Artificial Organs
Volume24
Issue number6
Publication statusPublished - 2001

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Keywords

  • Bioartificial liver
  • Hepatocyte
  • Species differences

ASJC Scopus subject areas

  • Biophysics

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