Abstract
Background: Platelet-rich plasma (PRP) has been advocated as one treatment for cartilage tissue regeneration. To date, several different platelet-rich formulations have been available, but a deep knowledge of their composition and mechanism of action in a specific clinical use is needed. The aim of this study was to investigate the effect of various PRP formulations on human chondrocytes in vitro. Methods: Blood from ten human volunteers was used to prepare three formulations: (1) PRP with a relatively low concentration of platelets and very few leukocytes (P-PRP), (2) PRP with high concentrations of both platelets and leukocytes (L-PRP), and (3) platelet-poor plasma (PPP). Selected growth factors in the formulations were measured, and the in vitro effects of various concentrations were tested by exposing chondrocytes isolated from osteoarthritic cartilage of four different men and measuring cell proliferation, matrix production, and gene expression. Results: L-PRP contained the highest levels of growth factors and cytokines. All three formulations stimulated chondrocyte proliferation throughout the culture period evaluated; the only significant difference among the formulations was on day 7, when P-PRP induced greater cell growth compared with the other two formulations. P-PRP stimulated chondrocyte anabolism, as shown by the expression of type-II collagen and aggrecan, whereas L-PRP promoted catabolic pathways involving various cytokines. However, L-PRP induced greater expression of the hyaluronic acid synthase-2 gene and greater production of hyaluronan compared with P-PRP. Conclusions: L-PRP and P-PRP induced distinct effects on human articular chondrocytes in vitro, possibly because of differences in the concentrations of platelets, leukocytes, growth factors, and other bioactive molecules. The identification of the optimal amounts and ratios of these blood components could ideally lead to a formulation more suitable for the treatment of cartilage lesions. Clinical Relevance: PRP formulations that are suitable for the treatment of degenerative joint diseases will likely contain balanced concentrations of platelets and leukocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 423-429 |
| Number of pages | 7 |
| Journal | Journal of Bone and Joint Surgery - Series A |
| Volume | 96 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 5 2014 |
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ASJC Scopus subject areas
- Surgery
- Orthopedics and Sports Medicine
Cite this
Comparison of platelet-rich plasma formulations for cartilage healing : An in vitro study. / Cavallo, Carola; Filardo, Giuseppe; Mariani, Erminia; Kon, Elizaveta; Marcacci, Maurilio; Pereira Ruiz, Maria Teresa; Facchini, Andrea; Grigolo, Brunella.
In: Journal of Bone and Joint Surgery - Series A, Vol. 96, No. 5, 05.03.2014, p. 423-429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of platelet-rich plasma formulations for cartilage healing
T2 - An in vitro study
AU - Cavallo, Carola
AU - Filardo, Giuseppe
AU - Mariani, Erminia
AU - Kon, Elizaveta
AU - Marcacci, Maurilio
AU - Pereira Ruiz, Maria Teresa
AU - Facchini, Andrea
AU - Grigolo, Brunella
PY - 2014/3/5
Y1 - 2014/3/5
N2 - Background: Platelet-rich plasma (PRP) has been advocated as one treatment for cartilage tissue regeneration. To date, several different platelet-rich formulations have been available, but a deep knowledge of their composition and mechanism of action in a specific clinical use is needed. The aim of this study was to investigate the effect of various PRP formulations on human chondrocytes in vitro. Methods: Blood from ten human volunteers was used to prepare three formulations: (1) PRP with a relatively low concentration of platelets and very few leukocytes (P-PRP), (2) PRP with high concentrations of both platelets and leukocytes (L-PRP), and (3) platelet-poor plasma (PPP). Selected growth factors in the formulations were measured, and the in vitro effects of various concentrations were tested by exposing chondrocytes isolated from osteoarthritic cartilage of four different men and measuring cell proliferation, matrix production, and gene expression. Results: L-PRP contained the highest levels of growth factors and cytokines. All three formulations stimulated chondrocyte proliferation throughout the culture period evaluated; the only significant difference among the formulations was on day 7, when P-PRP induced greater cell growth compared with the other two formulations. P-PRP stimulated chondrocyte anabolism, as shown by the expression of type-II collagen and aggrecan, whereas L-PRP promoted catabolic pathways involving various cytokines. However, L-PRP induced greater expression of the hyaluronic acid synthase-2 gene and greater production of hyaluronan compared with P-PRP. Conclusions: L-PRP and P-PRP induced distinct effects on human articular chondrocytes in vitro, possibly because of differences in the concentrations of platelets, leukocytes, growth factors, and other bioactive molecules. The identification of the optimal amounts and ratios of these blood components could ideally lead to a formulation more suitable for the treatment of cartilage lesions. Clinical Relevance: PRP formulations that are suitable for the treatment of degenerative joint diseases will likely contain balanced concentrations of platelets and leukocytes.
AB - Background: Platelet-rich plasma (PRP) has been advocated as one treatment for cartilage tissue regeneration. To date, several different platelet-rich formulations have been available, but a deep knowledge of their composition and mechanism of action in a specific clinical use is needed. The aim of this study was to investigate the effect of various PRP formulations on human chondrocytes in vitro. Methods: Blood from ten human volunteers was used to prepare three formulations: (1) PRP with a relatively low concentration of platelets and very few leukocytes (P-PRP), (2) PRP with high concentrations of both platelets and leukocytes (L-PRP), and (3) platelet-poor plasma (PPP). Selected growth factors in the formulations were measured, and the in vitro effects of various concentrations were tested by exposing chondrocytes isolated from osteoarthritic cartilage of four different men and measuring cell proliferation, matrix production, and gene expression. Results: L-PRP contained the highest levels of growth factors and cytokines. All three formulations stimulated chondrocyte proliferation throughout the culture period evaluated; the only significant difference among the formulations was on day 7, when P-PRP induced greater cell growth compared with the other two formulations. P-PRP stimulated chondrocyte anabolism, as shown by the expression of type-II collagen and aggrecan, whereas L-PRP promoted catabolic pathways involving various cytokines. However, L-PRP induced greater expression of the hyaluronic acid synthase-2 gene and greater production of hyaluronan compared with P-PRP. Conclusions: L-PRP and P-PRP induced distinct effects on human articular chondrocytes in vitro, possibly because of differences in the concentrations of platelets, leukocytes, growth factors, and other bioactive molecules. The identification of the optimal amounts and ratios of these blood components could ideally lead to a formulation more suitable for the treatment of cartilage lesions. Clinical Relevance: PRP formulations that are suitable for the treatment of degenerative joint diseases will likely contain balanced concentrations of platelets and leukocytes.
UR - http://www.scopus.com/inward/record.url?scp=84896369299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896369299&partnerID=8YFLogxK
U2 - 10.2106/JBJS.M.00726
DO - 10.2106/JBJS.M.00726
M3 - Article
C2 - 24599205
AN - SCOPUS:84896369299
VL - 96
SP - 423
EP - 429
JO - Journal of Bone and Joint Surgery - Series A
JF - Journal of Bone and Joint Surgery - Series A
SN - 0021-9355
IS - 5
ER -