Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients

Valeria Ghisetti, Anna Barbui, Enrico Donegani, Marco Bobbio, Philippe Caimmi, Stefano Pansini, Giuseppe Zattera, Angela Pucci, Michele Di Summa, Giovanna Marchiaro

Research output: Contribution to journalArticle

Abstract

Objective: To establish whether polymerase chain reaction (PCR) for cytomegalovirus deoxyribonucleic acid (DNA) can provide clinical information for the management of the infection. Methods: Leukocytes in 30 heart transplant recipients were monitored by pp65 antigen testing and PCR for 82 to 365 days after transplantation. Results: Of the 30 patients, 26 developed cytomegalovirus infection, nine of whom were symptomatic. Altogether, 300 leukocyte samples were examined. The concordance between PCR and pp65 antigen test was 82.6%. In symptomatic patients after surgery, PCR detected cytomegalovirus infection after 38 ± 16 days and the pp65 antigen test, after 48 ± 15 days. Symptomatic infection correlated with a higher number of pp65-positive leukocytes than did asymptomatic infection: 310 ± 356 vs 24 ± 35 (p <0.005)/200,000 examined, respectively. Clearance of virus was observed by PCR after 125 ± 73 days (range 29 to 225) in symptomatic, and after 82 ± 70 days (range 16 to 301) in asymptomatic, cases of infection. Conclusions: The positive predictive value of PCR for symptomatic infection was 34.6%. Our findings correlate with previous reports and show that the qualitative detection of cytomegalovirus DNA is not associated with overt disease whereas quantitation of pp65-positive leukocytes closely correlate with symptom onset. Insofar as the results are not quantitative, PCR is not a marker of clinically apparent infection. Careful monitoring of cytomegalovirus infection based on quantitative pp65 antigen assay can fulfill all clinical needs for early diagnosis and proper management of the infection.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalClinical Microbiology and Infection
Volume1
Issue number3
Publication statusPublished - Mar 1996

Fingerprint

Cytomegalovirus
Leukocytes
Antigens
Polymerase Chain Reaction
Cytomegalovirus Infections
Infection
Asymptomatic Infections
Information Management
DNA
Transplant Recipients
Early Diagnosis
Transplantation
Viruses

Keywords

  • Heart transplantation
  • Human cytomegalovirus (HCMV)
  • Polymerase chain reaction
  • pp65 antigen test

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients. / Ghisetti, Valeria; Barbui, Anna; Donegani, Enrico; Bobbio, Marco; Caimmi, Philippe; Pansini, Stefano; Zattera, Giuseppe; Pucci, Angela; Di Summa, Michele; Marchiaro, Giovanna.

In: Clinical Microbiology and Infection, Vol. 1, No. 3, 03.1996, p. 195-202.

Research output: Contribution to journalArticle

Ghisetti, V, Barbui, A, Donegani, E, Bobbio, M, Caimmi, P, Pansini, S, Zattera, G, Pucci, A, Di Summa, M & Marchiaro, G 1996, 'Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients', Clinical Microbiology and Infection, vol. 1, no. 3, pp. 195-202.
Ghisetti, Valeria ; Barbui, Anna ; Donegani, Enrico ; Bobbio, Marco ; Caimmi, Philippe ; Pansini, Stefano ; Zattera, Giuseppe ; Pucci, Angela ; Di Summa, Michele ; Marchiaro, Giovanna. / Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients. In: Clinical Microbiology and Infection. 1996 ; Vol. 1, No. 3. pp. 195-202.
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AU - Caimmi, Philippe

AU - Pansini, Stefano

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N2 - Objective: To establish whether polymerase chain reaction (PCR) for cytomegalovirus deoxyribonucleic acid (DNA) can provide clinical information for the management of the infection. Methods: Leukocytes in 30 heart transplant recipients were monitored by pp65 antigen testing and PCR for 82 to 365 days after transplantation. Results: Of the 30 patients, 26 developed cytomegalovirus infection, nine of whom were symptomatic. Altogether, 300 leukocyte samples were examined. The concordance between PCR and pp65 antigen test was 82.6%. In symptomatic patients after surgery, PCR detected cytomegalovirus infection after 38 ± 16 days and the pp65 antigen test, after 48 ± 15 days. Symptomatic infection correlated with a higher number of pp65-positive leukocytes than did asymptomatic infection: 310 ± 356 vs 24 ± 35 (p <0.005)/200,000 examined, respectively. Clearance of virus was observed by PCR after 125 ± 73 days (range 29 to 225) in symptomatic, and after 82 ± 70 days (range 16 to 301) in asymptomatic, cases of infection. Conclusions: The positive predictive value of PCR for symptomatic infection was 34.6%. Our findings correlate with previous reports and show that the qualitative detection of cytomegalovirus DNA is not associated with overt disease whereas quantitation of pp65-positive leukocytes closely correlate with symptom onset. Insofar as the results are not quantitative, PCR is not a marker of clinically apparent infection. Careful monitoring of cytomegalovirus infection based on quantitative pp65 antigen assay can fulfill all clinical needs for early diagnosis and proper management of the infection.

AB - Objective: To establish whether polymerase chain reaction (PCR) for cytomegalovirus deoxyribonucleic acid (DNA) can provide clinical information for the management of the infection. Methods: Leukocytes in 30 heart transplant recipients were monitored by pp65 antigen testing and PCR for 82 to 365 days after transplantation. Results: Of the 30 patients, 26 developed cytomegalovirus infection, nine of whom were symptomatic. Altogether, 300 leukocyte samples were examined. The concordance between PCR and pp65 antigen test was 82.6%. In symptomatic patients after surgery, PCR detected cytomegalovirus infection after 38 ± 16 days and the pp65 antigen test, after 48 ± 15 days. Symptomatic infection correlated with a higher number of pp65-positive leukocytes than did asymptomatic infection: 310 ± 356 vs 24 ± 35 (p <0.005)/200,000 examined, respectively. Clearance of virus was observed by PCR after 125 ± 73 days (range 29 to 225) in symptomatic, and after 82 ± 70 days (range 16 to 301) in asymptomatic, cases of infection. Conclusions: The positive predictive value of PCR for symptomatic infection was 34.6%. Our findings correlate with previous reports and show that the qualitative detection of cytomegalovirus DNA is not associated with overt disease whereas quantitation of pp65-positive leukocytes closely correlate with symptom onset. Insofar as the results are not quantitative, PCR is not a marker of clinically apparent infection. Careful monitoring of cytomegalovirus infection based on quantitative pp65 antigen assay can fulfill all clinical needs for early diagnosis and proper management of the infection.

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