Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis

Daniele Capitanio, Agnese Viganò, Enzo Ricci, Paolo Cerretelli, Robin Wait, Cecilia Gelfi

Research output: Contribution to journalArticlepeer-review

Abstract

We have used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to study the expression of contractile and regulatory proteins in human vastus lateralis and deltoideus muscles, in order to understand protein turnover and isoform switching in muscles with the same fiber-type composition but different functional properties. We demonstrate a two- to six-fold overexpression of enzymes associated with glycolysis, the tricarboxylic acid cycle, oxidative phosphorylation, and substrate transport in vastus lateralis compared to deltoideus. Expression levels of contractile protein isoforms correlated to the proportion of slow-twitch fibers in deltoideus compared to vastus lateralis are consistent with the different contractile properties of the two muscles. Two proteins involved in free radical homeostasis were differentially expressed, suggesting a direct relationship between radical scavenging and the muscle function. The application of 2-DE and MS to studies of muscle physiology thus offers a more comprehensive assessment of the molecular determinants of muscle function than traditional approaches.

Original languageEnglish
Pages (from-to)2577-2586
Number of pages10
JournalProteomics
Volume5
Issue number10
DOIs
Publication statusPublished - Jul 2005

Keywords

  • Fiber type
  • Human muscle
  • Mass spectrometry
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Comparison of protein expression in human deltoideus and vastus lateralis muscles using two-dimensional gel electrophoresis'. Together they form a unique fingerprint.

Cite this