Abstract
A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E coli antibodies from human sera are provided and their uses are discussed.
Original language | English |
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Pages (from-to) | 143-145 |
Number of pages | 3 |
Journal | Journal of Immunological Methods |
Volume | 190 |
Issue number | 1 |
DOIs | |
Publication status | Published - Mar 28 1996 |
Keywords
- Anti-E. call antibody depletion
- Autoantibody
- Immunoblotting
ASJC Scopus subject areas
- Biotechnology
- Immunology