Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

Giovanni Covini, Edward K L Chan, Massimo Colombo, E. M. Tan

Research output: Contribution to journalArticlepeer-review

Abstract

A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E coli antibodies from human sera are provided and their uses are discussed.

Original languageEnglish
Pages (from-to)143-145
Number of pages3
JournalJournal of Immunological Methods
Volume190
Issue number1
DOIs
Publication statusPublished - Mar 28 1996

Keywords

  • Anti-E. call antibody depletion
  • Autoantibody
  • Immunoblotting

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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