Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

Giovanni Covini; Edward K L Chan; Massimo Colombo; E. M. Tan

doi:10.1016/0022-1759(95)00288-X

Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

Giovanni Covini, Edward K L Chan, Massimo Colombo, E. M. Tan

Research output: Contribution to journal › Article › peer-review

Abstract

A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E coli antibodies from human sera are provided and their uses are discussed.

Original language	English
Pages (from-to)	143-145
Number of pages	3
Journal	Journal of Immunological Methods
Volume	190
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(95)00288-X
Publication status	Published - Mar 28 1996

Keywords

Anti-E. call antibody depletion
Autoantibody
Immunoblotting

ASJC Scopus subject areas

Biotechnology
Immunology

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10.1016/0022-1759(95)00288-X

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title = "Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens",

abstract = "A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E coli antibodies from human sera are provided and their uses are discussed.",

keywords = "Anti-E. call antibody depletion, Autoantibody, Immunoblotting",

author = "Giovanni Covini and Chan, {Edward K L} and Massimo Colombo and Tan, {E. M.}",

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KW - Immunoblotting

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