Comparison of radioimmunoassay and high-resolution gas chromatography mass spectrometry for the quantitative determination of serum thromboxane B2 and 6-keto-PGF1α after pharmacological blockade of thromboxane synthetase

C. Chiabrando, M. N. Castagnoli, A. Noseda, R. Fanelli, G. Rajtar, C. Cerletti, G. de Gaetano

Research output: Contribution to journalArticle

Abstract

Radioimmunoassay (RIA) and high-resolution gas chromatography-mass spectrometry (HRGC-MS) were compared for the determination of serum 6-keto-PGF- and TXB2 in a situation of drug-altered arachidonate metabolism. Results were comparable for TXB2 in both conditions. 6-keto-PGF RIA determinations (with two different antisera) revealed increased levels in dazoxiben-treated samples, which were not confirmed by HRGC-MS. The assay were repeatedly checked in controlled conditions to investigate these discrepancies. Interference was found with both antisera, due to the drug-induced change in metabolism.

Original languageEnglish
Pages (from-to)79-88
Number of pages10
JournalProstaglandins, Leukotrienes and Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

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