Comparison of real-time PCR methods for measurement of HIV-1 proviral DNA

Research output: Contribution to journalArticlepeer-review

Abstract

The use of HIV-1 DNA quantitation in cellular reservoirs to predict disease progression and treatment outcome in infected patients is hampered by the lack of standardization among the available methods. In the present study, real-time PCR methods used commonly for HIV-1 proviral DNA evaluation were compared, showing strong differences in the results, probably as a consequence of genome variability in the target regions. Standardization of HIV-1 proviral DNA quantitation assays is needed for use in clinical management of patients with HIV-1.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalJournal of Virological Methods
Volume164
Issue number1-2
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Marker
  • Proviral burden
  • Real-time PCR
  • Sequence

ASJC Scopus subject areas

  • Virology

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