Comparison of serological and molecular typing for HLA-A and -B on cord blood lymphocytes

F. Poli, M. Scalamogna, L. Crespiatico, B. Macchi, R. Mistò, A. Nocco, G. Rossini, C. Scarpino, V. Sioli, G. Sirchia

Research output: Contribution to journalArticlepeer-review

Abstract

HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing. PCR-SSP was successful in amplifying HLA-A,B alleles in all 100 cord blood samples. Forty-six typings gave discrepant results with the 2 methods (serology and PCR-SSP). Typings were considered discrepant also in the case of inability to define a split. For 19 specimens, no serological conclusion was drawn due to high mortality of the cell suspension, while PCR-SSP allowed the definition of a clear typing. In 6 cases it was necessary to infer information from serology to define the current typing. Finally, in 3 other cases it was impossible to exclude or attribute the antigen/allele B67 or B4802. PCR-SSP for HLA-A,B can improve the overall reliability of HLA-A,B typing requiring a small amount of blood although, with the set of sequence specific primers adopted, a number of alleles are still poorly defined.

Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalTissue Antigens
Volume51
Issue number1
Publication statusPublished - 1998

Keywords

  • Cord blood lymphocyte
  • Molecular typing
  • Serological typing

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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