Abstract
HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing. PCR-SSP was successful in amplifying HLA-A,B alleles in all 100 cord blood samples. Forty-six typings gave discrepant results with the 2 methods (serology and PCR-SSP). Typings were considered discrepant also in the case of inability to define a split. For 19 specimens, no serological conclusion was drawn due to high mortality of the cell suspension, while PCR-SSP allowed the definition of a clear typing. In 6 cases it was necessary to infer information from serology to define the current typing. Finally, in 3 other cases it was impossible to exclude or attribute the antigen/allele B67 or B4802. PCR-SSP for HLA-A,B can improve the overall reliability of HLA-A,B typing requiring a small amount of blood although, with the set of sequence specific primers adopted, a number of alleles are still poorly defined.
| Original language | English |
|---|---|
| Pages (from-to) | 67-71 |
| Number of pages | 5 |
| Journal | Tissue Antigens |
| Volume | 51 |
| Issue number | 1 |
| Publication status | Published - 1998 |
Fingerprint
Keywords
- Cord blood lymphocyte
- Molecular typing
- Serological typing
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
Comparison of serological and molecular typing for HLA-A and -B on cord blood lymphocytes. / Poli, F.; Scalamogna, M.; Crespiatico, L.; Macchi, B.; Mistò, R.; Nocco, A.; Rossini, G.; Scarpino, C.; Sioli, V.; Sirchia, G.
In: Tissue Antigens, Vol. 51, No. 1, 1998, p. 67-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of serological and molecular typing for HLA-A and -B on cord blood lymphocytes
AU - Poli, F.
AU - Scalamogna, M.
AU - Crespiatico, L.
AU - Macchi, B.
AU - Mistò, R.
AU - Nocco, A.
AU - Rossini, G.
AU - Scarpino, C.
AU - Sioli, V.
AU - Sirchia, G.
PY - 1998
Y1 - 1998
N2 - HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing. PCR-SSP was successful in amplifying HLA-A,B alleles in all 100 cord blood samples. Forty-six typings gave discrepant results with the 2 methods (serology and PCR-SSP). Typings were considered discrepant also in the case of inability to define a split. For 19 specimens, no serological conclusion was drawn due to high mortality of the cell suspension, while PCR-SSP allowed the definition of a clear typing. In 6 cases it was necessary to infer information from serology to define the current typing. Finally, in 3 other cases it was impossible to exclude or attribute the antigen/allele B67 or B4802. PCR-SSP for HLA-A,B can improve the overall reliability of HLA-A,B typing requiring a small amount of blood although, with the set of sequence specific primers adopted, a number of alleles are still poorly defined.
AB - HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing. PCR-SSP was successful in amplifying HLA-A,B alleles in all 100 cord blood samples. Forty-six typings gave discrepant results with the 2 methods (serology and PCR-SSP). Typings were considered discrepant also in the case of inability to define a split. For 19 specimens, no serological conclusion was drawn due to high mortality of the cell suspension, while PCR-SSP allowed the definition of a clear typing. In 6 cases it was necessary to infer information from serology to define the current typing. Finally, in 3 other cases it was impossible to exclude or attribute the antigen/allele B67 or B4802. PCR-SSP for HLA-A,B can improve the overall reliability of HLA-A,B typing requiring a small amount of blood although, with the set of sequence specific primers adopted, a number of alleles are still poorly defined.
KW - Cord blood lymphocyte
KW - Molecular typing
KW - Serological typing
UR - http://www.scopus.com/inward/record.url?scp=15144351067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15144351067&partnerID=8YFLogxK
M3 - Article
C2 - 9459505
AN - SCOPUS:15144351067
VL - 51
SP - 67
EP - 71
JO - Tissue Antigens
JF - Tissue Antigens
SN - 0001-2815
IS - 1
ER -