Comparison of the artus HIV-1 QS-RGQ and VERSANT HIV-1 RNA 1.0 assays for quantitative detection of human immunodeficiency virus type 1 in plasma samples

Roee Dvir, Filippo Canducci, Sara Racca, Serena Rolla, Sonia Stucchi, Massimo Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

Several integrated diagnostic platforms to quantify human immunodeficiency virus type-1 viremia have been developed in recent years. We evaluated the performances of the Artus HIV-1 QS-RGQ assay, using the complete QIAsymphony RGQ workflow. 192 clinical plasma specimens and external control panel samples were analyzed, using the Artus assay and the routine Siemens VERSANT HIV-1 RNA 1.0 assay. Three samples were excluded due to amplification inhibition. Among the remaining 189 specimens, 130 samples were detected as positive (above the limit of detection by both assays; median log10 difference: 0.01) and 18 samples were detected as negative. Eight samples (4.2%), all slightly above the limit of detection of the Versant assay, were negative with the Artus assay. The remaining 33 samples (beside 3 negative by Artus assay) were positive by both assays, but below the limit of detection at least in one of them. Results from the external panel samples showed a mean Log10 variation of -0.18 and -0.45 for the Versant and the Artus assays, respectively. As both assays showed highly correlated results, the QIAsymphony RGQ system, using the Artus HIV-1 QS-RGQ assay, could be considered a potential platform for HIV-1 RNA quantification in plasma.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalNew Microbiologica
Volume38
Issue number3
Publication statusPublished - Jul 1 2015

Keywords

  • Artus HIV-1 QS-RGQ assay
  • Human immunodeficiency virus type-1 (HIV-1)
  • Quantitative real-time polymerase chain reaction (qPCR)

ASJC Scopus subject areas

  • Microbiology (medical)
  • Medicine(all)

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