TY - JOUR
T1 - Comparison of the breakpoint regions of ELE1 and RET genes involved in the generation of RET/PTC3 oncogene in sporadic and in radiation-associated papillary thyroid carcinomas
AU - Bongarzone, Italia
AU - Butti, Marta G.
AU - Fugazzola, Laura
AU - Pacini, Furio
AU - Pinchera, Aldo
AU - Vorontsova, Tatiana V.
AU - Demidchik, Eugene P.
AU - Pierotti, Marco A.
PY - 1997/6/1
Y1 - 1997/6/1
N2 - The RET/PTC3 oncogene is an activated form of the RET protooncogene, which is frequently rearranged in papillary thyroid carcinoma. RET/PTC3 results from a structural rearrangement between the ELE1 and the RET genes, and it has been observed in both sporadic and radiation-associated post- Chernobyl tumors. To understand the molecular basis that predisposes RET and ELE1 genes to be recurrent targets of 'illegitimate' recombination, we examined the genomic regions containing the ELE1/RET breakpoints of six sporadic and three post-Chernobyl tumors in two papillary carcinomas of different origins. Our data indicated, in both genes, a clustering of the breakpoints in regions designated ELE1-bcr (1.8 kb) and RET-bcr (1.9 kb). Notably, in all sporadic tumors and in one post-Chernobyl tumor the ELE1/RET recombination corresponded with short sequences of homology (3-7 nt) between the two rearranging genes. In addition, we observed an interesting distribution of the post-Chernobyl breakpoints in ELE1-bcr located within an Alu element, or in between two close Alu elements, and always in A+T-rich regions.
AB - The RET/PTC3 oncogene is an activated form of the RET protooncogene, which is frequently rearranged in papillary thyroid carcinoma. RET/PTC3 results from a structural rearrangement between the ELE1 and the RET genes, and it has been observed in both sporadic and radiation-associated post- Chernobyl tumors. To understand the molecular basis that predisposes RET and ELE1 genes to be recurrent targets of 'illegitimate' recombination, we examined the genomic regions containing the ELE1/RET breakpoints of six sporadic and three post-Chernobyl tumors in two papillary carcinomas of different origins. Our data indicated, in both genes, a clustering of the breakpoints in regions designated ELE1-bcr (1.8 kb) and RET-bcr (1.9 kb). Notably, in all sporadic tumors and in one post-Chernobyl tumor the ELE1/RET recombination corresponded with short sequences of homology (3-7 nt) between the two rearranging genes. In addition, we observed an interesting distribution of the post-Chernobyl breakpoints in ELE1-bcr located within an Alu element, or in between two close Alu elements, and always in A+T-rich regions.
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U2 - 10.1006/geno.1997.4685
DO - 10.1006/geno.1997.4685
M3 - Article
C2 - 9192845
AN - SCOPUS:0031172072
VL - 42
SP - 252
EP - 259
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -