Comparison of the colony-forming capacities of human T-lymphocyte subpopulations

Vito Pistoia, Riccardo Ghio, G. Walter Canonica, Marco Colombatti, Lorenzo Moretta

Research output: Contribution to journalArticlepeer-review

Abstract

Purified human peripheral blood T cells were fractionated into cells with IgM receptors (TM) or IgG receptors (TG) or "null" cells, i.e., cells without detectable Fc μ and Fc γ receptors (T null), and tested for colony formation in semisolid medium in the presence of PHA. TG cells formed only a few colonies, TM cells had the same efficiency as unfractionated T lymphocytes, while the T null fraction consistently showed the highest cloning capacity. The addition of adherent cells (AC) enhanced the cloning efficiency of purified T cells and of all of the subsets. However, the presence of AC was not a strict requirement for colony induction nor did it eliminate the observed differences in the colony-forming capacities of the various subsets.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalClinical Immunology and Immunopathology
Volume21
Issue number3
DOIs
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Comparison of the colony-forming capacities of human T-lymphocyte subpopulations'. Together they form a unique fingerprint.

Cite this