Abstract
Purified human peripheral blood T cells were fractionated into cells with IgM receptors (TM) or IgG receptors (TG) or "null" cells, i.e., cells without detectable Fc μ and Fc γ receptors (T null), and tested for colony formation in semisolid medium in the presence of PHA. TG cells formed only a few colonies, TM cells had the same efficiency as unfractionated T lymphocytes, while the T null fraction consistently showed the highest cloning capacity. The addition of adherent cells (AC) enhanced the cloning efficiency of purified T cells and of all of the subsets. However, the presence of AC was not a strict requirement for colony induction nor did it eliminate the observed differences in the colony-forming capacities of the various subsets.
| Original language | English |
|---|---|
| Pages (from-to) | 289-294 |
| Number of pages | 6 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 21 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1981 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
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Comparison of the colony-forming capacities of human T-lymphocyte subpopulations. / Pistoia, Vito; Ghio, Riccardo; Canonica, G. Walter; Colombatti, Marco; Moretta, Lorenzo.
In: Clinical Immunology and Immunopathology, Vol. 21, No. 3, 1981, p. 289-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of the colony-forming capacities of human T-lymphocyte subpopulations
AU - Pistoia, Vito
AU - Ghio, Riccardo
AU - Canonica, G. Walter
AU - Colombatti, Marco
AU - Moretta, Lorenzo
PY - 1981
Y1 - 1981
N2 - Purified human peripheral blood T cells were fractionated into cells with IgM receptors (TM) or IgG receptors (TG) or "null" cells, i.e., cells without detectable Fc μ and Fc γ receptors (T null), and tested for colony formation in semisolid medium in the presence of PHA. TG cells formed only a few colonies, TM cells had the same efficiency as unfractionated T lymphocytes, while the T null fraction consistently showed the highest cloning capacity. The addition of adherent cells (AC) enhanced the cloning efficiency of purified T cells and of all of the subsets. However, the presence of AC was not a strict requirement for colony induction nor did it eliminate the observed differences in the colony-forming capacities of the various subsets.
AB - Purified human peripheral blood T cells were fractionated into cells with IgM receptors (TM) or IgG receptors (TG) or "null" cells, i.e., cells without detectable Fc μ and Fc γ receptors (T null), and tested for colony formation in semisolid medium in the presence of PHA. TG cells formed only a few colonies, TM cells had the same efficiency as unfractionated T lymphocytes, while the T null fraction consistently showed the highest cloning capacity. The addition of adherent cells (AC) enhanced the cloning efficiency of purified T cells and of all of the subsets. However, the presence of AC was not a strict requirement for colony induction nor did it eliminate the observed differences in the colony-forming capacities of the various subsets.
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U2 - 10.1016/0090-1229(81)90217-8
DO - 10.1016/0090-1229(81)90217-8
M3 - Article
VL - 21
SP - 289
EP - 294
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
SN - 0090-1229
IS - 3
ER -