Comparison of the colony-forming capacities of human T-lymphocyte subpopulations

Vito Pistoia, Riccardo Ghio, G. Walter Canonica, Marco Colombatti, Lorenzo Moretta

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purified human peripheral blood T cells were fractionated into cells with IgM receptors (TM) or IgG receptors (TG) or "null" cells, i.e., cells without detectable Fc μ and Fc γ receptors (T null), and tested for colony formation in semisolid medium in the presence of PHA. TG cells formed only a few colonies, TM cells had the same efficiency as unfractionated T lymphocytes, while the T null fraction consistently showed the highest cloning capacity. The addition of adherent cells (AC) enhanced the cloning efficiency of purified T cells and of all of the subsets. However, the presence of AC was not a strict requirement for colony induction nor did it eliminate the observed differences in the colony-forming capacities of the various subsets.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalClinical Immunology and Immunopathology
Volume21
Issue number3
DOIs
Publication statusPublished - 1981

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Lymphocyte Subsets
T-Lymphocytes
Organism Cloning
IgG Receptors
Null Lymphocytes
Fc Receptors
T-Lymphocyte Subsets
Immunoglobulin M
Blood Cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Comparison of the colony-forming capacities of human T-lymphocyte subpopulations. / Pistoia, Vito; Ghio, Riccardo; Canonica, G. Walter; Colombatti, Marco; Moretta, Lorenzo.

In: Clinical Immunology and Immunopathology, Vol. 21, No. 3, 1981, p. 289-294.

Research output: Contribution to journalArticle

Pistoia, Vito ; Ghio, Riccardo ; Canonica, G. Walter ; Colombatti, Marco ; Moretta, Lorenzo. / Comparison of the colony-forming capacities of human T-lymphocyte subpopulations. In: Clinical Immunology and Immunopathology. 1981 ; Vol. 21, No. 3. pp. 289-294.
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