Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines

Fabiola Rusolo, Francesca Capone, Raffaella Pasquale, Antonella Angiolillo, Giovanni Colonna, Giuseppe Castello, Maria Costantini, Susan Costantini

Research output: Contribution to journalArticle

Abstract

Breast cancer is the second most common cause of mortality in women; therefore, the identification of novel putative markers is required to improve its diagnosis and prognosis. Selenium is known to protect mammary epithelial cells from oxidative DNA damage, and to inhibit the initiation phase of carcinogenesis by stimulating DNA repair and apoptosis regulation. Consequently, the present study has focused attention on the selenoprotein family and their involvement in breast cancer. The present study performed a global analysis of the seleno-transcriptome expression in human breast cancer MCF-7 and MDA-MB231 cell lines compared with healthy breast MCF-10A cells using reverse transcription-quantitative polymerase chain reaction. The present data revealed the presence of differently expressed genes in MCF-7 and MDA-MB231 cells compared with MCF-10A cells: Four downregulated [glutathione peroxidase (GPX)1, GPX4, GPX5 and GPX7] and three upregulated (deiodinase iodothyronine, type II, GPX2 and GPX3) genes. Additionally, interactomic investigation were performed by the present study to evaluate the association between the downregulated and upregulated genes, and to identify putative HUB nodes, which represent the centers of association between the genes that are capable of direct control over the gene networks. Network analysis revealed that all differentially regulated genes, with the exception of selenoprotein T, are implicated in the same network that presents three HUB nodes interconnected to the selenoprotein mRNAs, including TP53, estrogen receptor 1 and catenin-β1 (CTNNB1). Overall, these data demonstrated for the first time, a profile of seleno-mRNAs specific for human breast cells, indicating that these genes alter their expression on the basis of the ER-positivity or negativity of breast cancer cells.

Original languageEnglish
Pages (from-to)2411-2417
Number of pages7
JournalOncology Letters
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Transcriptome
Breast
Epithelial Cells
Selenoproteins
Breast Neoplasms
Cell Line
Genes
phospholipid-hydroperoxide glutathione peroxidase
Down-Regulation
Catenins
Messenger RNA
Estrogen Receptor alpha
Gene Regulatory Networks
Gene Expression Profiling
Selenium
DNA Repair
DNA Damage
Reverse Transcription
Carcinogenesis
Apoptosis

Keywords

  • Breast cancer
  • HUB genes
  • Network analysis
  • RT-qPCR
  • Seleno-transcriptome

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Rusolo, F., Capone, F., Pasquale, R., Angiolillo, A., Colonna, G., Castello, G., ... Costantini, S. (2017). Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines. Oncology Letters, 13(4), 2411-2417. https://doi.org/10.3892/ol.2017.5715

Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines. / Rusolo, Fabiola; Capone, Francesca; Pasquale, Raffaella; Angiolillo, Antonella; Colonna, Giovanni; Castello, Giuseppe; Costantini, Maria; Costantini, Susan.

In: Oncology Letters, Vol. 13, No. 4, 01.04.2017, p. 2411-2417.

Research output: Contribution to journalArticle

Rusolo, F, Capone, F, Pasquale, R, Angiolillo, A, Colonna, G, Castello, G, Costantini, M & Costantini, S 2017, 'Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines', Oncology Letters, vol. 13, no. 4, pp. 2411-2417. https://doi.org/10.3892/ol.2017.5715
Rusolo, Fabiola ; Capone, Francesca ; Pasquale, Raffaella ; Angiolillo, Antonella ; Colonna, Giovanni ; Castello, Giuseppe ; Costantini, Maria ; Costantini, Susan. / Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines. In: Oncology Letters. 2017 ; Vol. 13, No. 4. pp. 2411-2417.
@article{4fa421d3f7564211a3d7bb9f889fa5f1,
title = "Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines",
abstract = "Breast cancer is the second most common cause of mortality in women; therefore, the identification of novel putative markers is required to improve its diagnosis and prognosis. Selenium is known to protect mammary epithelial cells from oxidative DNA damage, and to inhibit the initiation phase of carcinogenesis by stimulating DNA repair and apoptosis regulation. Consequently, the present study has focused attention on the selenoprotein family and their involvement in breast cancer. The present study performed a global analysis of the seleno-transcriptome expression in human breast cancer MCF-7 and MDA-MB231 cell lines compared with healthy breast MCF-10A cells using reverse transcription-quantitative polymerase chain reaction. The present data revealed the presence of differently expressed genes in MCF-7 and MDA-MB231 cells compared with MCF-10A cells: Four downregulated [glutathione peroxidase (GPX)1, GPX4, GPX5 and GPX7] and three upregulated (deiodinase iodothyronine, type II, GPX2 and GPX3) genes. Additionally, interactomic investigation were performed by the present study to evaluate the association between the downregulated and upregulated genes, and to identify putative HUB nodes, which represent the centers of association between the genes that are capable of direct control over the gene networks. Network analysis revealed that all differentially regulated genes, with the exception of selenoprotein T, are implicated in the same network that presents three HUB nodes interconnected to the selenoprotein mRNAs, including TP53, estrogen receptor 1 and catenin-β1 (CTNNB1). Overall, these data demonstrated for the first time, a profile of seleno-mRNAs specific for human breast cells, indicating that these genes alter their expression on the basis of the ER-positivity or negativity of breast cancer cells.",
keywords = "Breast cancer, HUB genes, Network analysis, RT-qPCR, Seleno-transcriptome",
author = "Fabiola Rusolo and Francesca Capone and Raffaella Pasquale and Antonella Angiolillo and Giovanni Colonna and Giuseppe Castello and Maria Costantini and Susan Costantini",
year = "2017",
month = "4",
day = "1",
doi = "10.3892/ol.2017.5715",
language = "English",
volume = "13",
pages = "2411--2417",
journal = "Oncology Letters",
issn = "1792-1074",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines

AU - Rusolo, Fabiola

AU - Capone, Francesca

AU - Pasquale, Raffaella

AU - Angiolillo, Antonella

AU - Colonna, Giovanni

AU - Castello, Giuseppe

AU - Costantini, Maria

AU - Costantini, Susan

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Breast cancer is the second most common cause of mortality in women; therefore, the identification of novel putative markers is required to improve its diagnosis and prognosis. Selenium is known to protect mammary epithelial cells from oxidative DNA damage, and to inhibit the initiation phase of carcinogenesis by stimulating DNA repair and apoptosis regulation. Consequently, the present study has focused attention on the selenoprotein family and their involvement in breast cancer. The present study performed a global analysis of the seleno-transcriptome expression in human breast cancer MCF-7 and MDA-MB231 cell lines compared with healthy breast MCF-10A cells using reverse transcription-quantitative polymerase chain reaction. The present data revealed the presence of differently expressed genes in MCF-7 and MDA-MB231 cells compared with MCF-10A cells: Four downregulated [glutathione peroxidase (GPX)1, GPX4, GPX5 and GPX7] and three upregulated (deiodinase iodothyronine, type II, GPX2 and GPX3) genes. Additionally, interactomic investigation were performed by the present study to evaluate the association between the downregulated and upregulated genes, and to identify putative HUB nodes, which represent the centers of association between the genes that are capable of direct control over the gene networks. Network analysis revealed that all differentially regulated genes, with the exception of selenoprotein T, are implicated in the same network that presents three HUB nodes interconnected to the selenoprotein mRNAs, including TP53, estrogen receptor 1 and catenin-β1 (CTNNB1). Overall, these data demonstrated for the first time, a profile of seleno-mRNAs specific for human breast cells, indicating that these genes alter their expression on the basis of the ER-positivity or negativity of breast cancer cells.

AB - Breast cancer is the second most common cause of mortality in women; therefore, the identification of novel putative markers is required to improve its diagnosis and prognosis. Selenium is known to protect mammary epithelial cells from oxidative DNA damage, and to inhibit the initiation phase of carcinogenesis by stimulating DNA repair and apoptosis regulation. Consequently, the present study has focused attention on the selenoprotein family and their involvement in breast cancer. The present study performed a global analysis of the seleno-transcriptome expression in human breast cancer MCF-7 and MDA-MB231 cell lines compared with healthy breast MCF-10A cells using reverse transcription-quantitative polymerase chain reaction. The present data revealed the presence of differently expressed genes in MCF-7 and MDA-MB231 cells compared with MCF-10A cells: Four downregulated [glutathione peroxidase (GPX)1, GPX4, GPX5 and GPX7] and three upregulated (deiodinase iodothyronine, type II, GPX2 and GPX3) genes. Additionally, interactomic investigation were performed by the present study to evaluate the association between the downregulated and upregulated genes, and to identify putative HUB nodes, which represent the centers of association between the genes that are capable of direct control over the gene networks. Network analysis revealed that all differentially regulated genes, with the exception of selenoprotein T, are implicated in the same network that presents three HUB nodes interconnected to the selenoprotein mRNAs, including TP53, estrogen receptor 1 and catenin-β1 (CTNNB1). Overall, these data demonstrated for the first time, a profile of seleno-mRNAs specific for human breast cells, indicating that these genes alter their expression on the basis of the ER-positivity or negativity of breast cancer cells.

KW - Breast cancer

KW - HUB genes

KW - Network analysis

KW - RT-qPCR

KW - Seleno-transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85015275412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015275412&partnerID=8YFLogxK

U2 - 10.3892/ol.2017.5715

DO - 10.3892/ol.2017.5715

M3 - Article

AN - SCOPUS:85015275412

VL - 13

SP - 2411

EP - 2417

JO - Oncology Letters

JF - Oncology Letters

SN - 1792-1074

IS - 4

ER -