Comparison of three different tests for assessment of hepatitis C virus in dialysis patients

C. Casteinovo, G. Lunghi, A. De Vecchi, A. Grancini, G. Como, G. Graziani, A. Scalamogna, C. Ponticelli

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the relationship between hepatitis C virus antibodies (HCV-Ab) and viremia and to compare the prevalence of HCV-Ab and HCV viremia in hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients. Design: Cross-sectional study. Setting: Dialysis unit of a nephrology division in a public university hospital. Patients: All dialysis patients who came for routine clinic visits during the study period. None denied informed consent. Forty-eight patients on HD and 79 on CAPD were examined. Intervention: Blood samples were tested by second-generation enzyme-linked immunosorbent assay (ELISA II) and recombinant immunoblot assay (RIBA II) to look for HCV-Ab and by the polymerase chain reaction (PCR) to look for HCV viremia. Results: ELISA II was positive in 52% of HD patients and in 14% of CAPD patients. RIBA II was positive in 48% of HD patients and in 11% of CAPD patients. HCV viremia was positive by PCR in 41.6% of HD patients and in 12% of CAPD patients. Two of these PCR-positive patients did not show HCV-Ab by ELISA II and RIBA II. The sensitivity and specificity of ELISA II were 93% and 92%, the sensitivity and specificity of RIBA II were 86% and 94%. Conclusions: Our data confirm a higher prevalence of HCV viremia in HD than in CAPD patients. The absence of Ab against virus C in 2 patients positive with PCR might be due to recent HCV infection or to weak virus replication or to a poor immune response.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalPeritoneal Dialysis International
Volume15
Issue number6
Publication statusPublished - 1995

Keywords

  • Hepatitis C virus
  • Liver infections
  • Viral hepatitis

ASJC Scopus subject areas

  • Nephrology

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