Comparison of two immunoassays for the complement protein C4b-binding protein in health and disease

R. Coppola, S. Tombesi, P. Cristilli, L. Bergamaschini, P. M. Mannucci

Research output: Contribution to journalArticle

Abstract

An enzyme-linked immunoassay for human C4b-binding protein (concentration range 25-400 ng/ml) was developed using two monoclonal antibodies; the intra- and interassay coefficients of variation were less than 7.2%. In 50 normal subjects, 20 patients with liver cirrhosis and 34 full-term newborns, the plasma levels of C4b-binding protein were very similar to those measured by Laurell electroimmunoassay; in 24 patients with elevated erythrocyte sedimentation rates, levels measured by enzyme-linked immunoassay were higher then those measured by the Laurell method (270±70% vs. 223±67%). In these patients crossed immunoelectrophoresis showed a pattern different from that of normal individuals, which may explain the lower values found with the Laurell method.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalInternational Journal of Clinical & Laboratory Research
Volume25
Issue number2
DOIs
Publication statusPublished - Jun 1995

Keywords

  • C4BP
  • ELISA
  • Laurell

ASJC Scopus subject areas

  • Clinical Biochemistry

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