Amiloride, a diuretic and antihypertensive drug, inhibits Na + transporting systems, diamine oxidases and the human urinary plasminogen activator. Present results indicate that amiloride is a competitive inhibitor of mouse brain nitric oxide synthase (NOS; K(i) = 4.5 x 10 -4 M, at pH 7.5 and 37.0°C), but not a nitric oxide (NO) precursor, representing a case for enzyme cross-inhibition. The decreased levels of NO, as a consequence of NOS inhibition, might induce some clinically-adverse amiloride reactions, such as an unexpected reduced antihypertensive effect.
|Number of pages||5|
|Journal||Biochemistry and Molecular Biology International|
|Publication status||Published - Oct 1997|
- Mouse brain nitric oxide synthase
- Nitric oxide synthase inhibition
ASJC Scopus subject areas
- Molecular Biology