Complement dependent serum cytotoxicity of cancer patients studied by 51Cr release assay on human cancer lines

J. G. Puche, S. Canevari, G. Fossati, G. D. Porta, P. Vezzoni

Research output: Contribution to journalArticle

Abstract

The conditions for a 51Cr release assay on cell lines derived from human solid tumors were determined by using a rabbit antihuman antiserum with human AB serum as source of complement. By this assay, 38 sera collected before and after surgery from 19 breast cancer patients and 28 sera from 12 melanoma patients and 16 healthy donors were tested on 3 lines derived from breast cancer tissue and 1 line derived from normal breast tissue. Ten of the breast cancer patients had lymph node metastases and 9 did not. Positive reactions were obtained from 4 breast cancer patients, all with lymph node metastases, and from 1 healthy donor. In a second experiment, 58 coded sera belonging to 4 different groups of cancer and non-cancer individuals were assayed on a colonic cancer line (HT-29), and 33 and 20 of them were also tested on a melanoma line (MeWo) and on a breast cancer line (MaCa 13), respectively. Positive responses were few, and were more frequently observed among transfused than non-transfused patients.

Original languageEnglish
Pages (from-to)97-108
Number of pages12
JournalTumori
Volume63
Issue number1
Publication statusPublished - 1977

Fingerprint

Breast Neoplasms
Serum
Neoplasms
Melanoma
Lepidium
Lymph Nodes
Tissue Donors
Neoplasm Metastasis
Colonic Neoplasms
Immune Sera
Breast
Rabbits
Cell Line

ASJC Scopus subject areas

  • Cancer Research

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Complement dependent serum cytotoxicity of cancer patients studied by 51Cr release assay on human cancer lines. / Puche, J. G.; Canevari, S.; Fossati, G.; Porta, G. D.; Vezzoni, P.

In: Tumori, Vol. 63, No. 1, 1977, p. 97-108.

Research output: Contribution to journalArticle

Puche, J. G. ; Canevari, S. ; Fossati, G. ; Porta, G. D. ; Vezzoni, P. / Complement dependent serum cytotoxicity of cancer patients studied by 51Cr release assay on human cancer lines. In: Tumori. 1977 ; Vol. 63, No. 1. pp. 97-108.
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