Abstract
The complement-fixation test may be used to detect rotaviral antigens directly in clinical specimens. However, a certain number of specimens tested for human rotaviruses by the complementfixation test show an anti-complementary activity. By comparing eight techniques we analysed this anti-complementary activity and identified the best method for its reduction. Pretreatment of clarified supernatant of stool suspensions by some methods resulted in a reduction of anti-complementary activity, without reducing the sensitivity of the method. Clarified supernatants of 8 36 (22.2%) specimens were anti-complementary; this anti-complementary activity was best removed by absorption with fetal calf serum or calf albumin. Such treatment offers practical means of increasing the specificity of complement-fixation test. Some observations suggest that the anti-complementary activity of stool suspensions may be frequently due to the presence of one or more chelating agents that may be in faecal specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 329-335 |
| Number of pages | 7 |
| Journal | Journal of Virological Methods |
| Volume | 3 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1982 |
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Keywords
- anti-complementary activity
- complement fixation test
- rotavirus detection
ASJC Scopus subject areas
- Virology
Cite this
Complement-fixation test for rotavirus detection : comparison and analysis of different methods to reduce anti-complementary activity of some specimens. / Pauri, Paola; Bagnarelli, Patrizia; Clementi, Massimo.
In: Journal of Virological Methods, Vol. 3, No. 6, 1982, p. 329-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Complement-fixation test for rotavirus detection
T2 - comparison and analysis of different methods to reduce anti-complementary activity of some specimens
AU - Pauri, Paola
AU - Bagnarelli, Patrizia
AU - Clementi, Massimo
PY - 1982
Y1 - 1982
N2 - The complement-fixation test may be used to detect rotaviral antigens directly in clinical specimens. However, a certain number of specimens tested for human rotaviruses by the complementfixation test show an anti-complementary activity. By comparing eight techniques we analysed this anti-complementary activity and identified the best method for its reduction. Pretreatment of clarified supernatant of stool suspensions by some methods resulted in a reduction of anti-complementary activity, without reducing the sensitivity of the method. Clarified supernatants of 8 36 (22.2%) specimens were anti-complementary; this anti-complementary activity was best removed by absorption with fetal calf serum or calf albumin. Such treatment offers practical means of increasing the specificity of complement-fixation test. Some observations suggest that the anti-complementary activity of stool suspensions may be frequently due to the presence of one or more chelating agents that may be in faecal specimens.
AB - The complement-fixation test may be used to detect rotaviral antigens directly in clinical specimens. However, a certain number of specimens tested for human rotaviruses by the complementfixation test show an anti-complementary activity. By comparing eight techniques we analysed this anti-complementary activity and identified the best method for its reduction. Pretreatment of clarified supernatant of stool suspensions by some methods resulted in a reduction of anti-complementary activity, without reducing the sensitivity of the method. Clarified supernatants of 8 36 (22.2%) specimens were anti-complementary; this anti-complementary activity was best removed by absorption with fetal calf serum or calf albumin. Such treatment offers practical means of increasing the specificity of complement-fixation test. Some observations suggest that the anti-complementary activity of stool suspensions may be frequently due to the presence of one or more chelating agents that may be in faecal specimens.
KW - anti-complementary activity
KW - complement fixation test
KW - rotavirus detection
UR - http://www.scopus.com/inward/record.url?scp=0019832651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019832651&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(82)90037-4
DO - 10.1016/0166-0934(82)90037-4
M3 - Article
VL - 3
SP - 329
EP - 335
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 6
ER -