Abstract
Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.
| Original language | English |
|---|---|
| Pages (from-to) | 15-21 |
| Number of pages | 7 |
| Journal | Histochemistry |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1979 |
Fingerprint
ASJC Scopus subject areas
- Anatomy
Cite this
Complement-mediated unspecific binding of immunoglobulins to some endocrine cells. / Buffa, R.; Crivelli, O.; Fiocca, R.; Fontana, P.; Solcia, E.
In: Histochemistry, Vol. 63, No. 1, 01.1979, p. 15-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Complement-mediated unspecific binding of immunoglobulins to some endocrine cells
AU - Buffa, R.
AU - Crivelli, O.
AU - Fiocca, R.
AU - Fontana, P.
AU - Solcia, E.
PY - 1979/1
Y1 - 1979/1
N2 - Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.
AB - Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.
UR - http://www.scopus.com/inward/record.url?scp=0018736234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018736234&partnerID=8YFLogxK
U2 - 10.1007/BF00508008
DO - 10.1007/BF00508008
M3 - Article
C2 - 389892
AN - SCOPUS:0018736234
VL - 63
SP - 15
EP - 21
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 1
ER -