Complement-mediated unspecific binding of immunoglobulins to some endocrine cells

R. Buffa, O. Crivelli, R. Fiocca, P. Fontana, E. Solcia

Research output: Contribution to journalArticle

Abstract

Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalHistochemistry
Volume63
Issue number1
DOIs
Publication statusPublished - Jan 1979

Fingerprint

Endocrine Cells
Somatostatin-Secreting Cells
Immunoglobulins
Gastrin-Secreting Cells
Complement C1q
Hormones
Calcitonin
Gastric Mucosa
Glucagon
Pancreas
Anti-Idiotypic Antibodies
Thyroid Gland
Immunohistochemistry
Rabbits
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • Anatomy

Cite this

Complement-mediated unspecific binding of immunoglobulins to some endocrine cells. / Buffa, R.; Crivelli, O.; Fiocca, R.; Fontana, P.; Solcia, E.

In: Histochemistry, Vol. 63, No. 1, 01.1979, p. 15-21.

Research output: Contribution to journalArticle

Buffa, R. ; Crivelli, O. ; Fiocca, R. ; Fontana, P. ; Solcia, E. / Complement-mediated unspecific binding of immunoglobulins to some endocrine cells. In: Histochemistry. 1979 ; Vol. 63, No. 1. pp. 15-21.
@article{9b6b866ec6d64c179104b828c5a42ed7,
title = "Complement-mediated unspecific binding of immunoglobulins to some endocrine cells",
abstract = "Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.",
author = "R. Buffa and O. Crivelli and R. Fiocca and P. Fontana and E. Solcia",
year = "1979",
month = "1",
doi = "10.1007/BF00508008",
language = "English",
volume = "63",
pages = "15--21",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Complement-mediated unspecific binding of immunoglobulins to some endocrine cells

AU - Buffa, R.

AU - Crivelli, O.

AU - Fiocca, R.

AU - Fontana, P.

AU - Solcia, E.

PY - 1979/1

Y1 - 1979/1

N2 - Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.

AB - Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.

UR - http://www.scopus.com/inward/record.url?scp=0018736234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018736234&partnerID=8YFLogxK

U2 - 10.1007/BF00508008

DO - 10.1007/BF00508008

M3 - Article

C2 - 389892

AN - SCOPUS:0018736234

VL - 63

SP - 15

EP - 21

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 1

ER -