TY - JOUR
T1 - Complementary function of γδ T-lymphocytes and dendritic cells in the response to isopentenyl-pyrophosphate and lipopolysaccharide antigens
AU - Martino, Angelo
AU - Casetti, Rita
AU - D'Alessandri, Alessandra
AU - Sacchi, Alessandra
AU - Poccia, Fabrizio
PY - 2005/5
Y1 - 2005/5
N2 - Dendritic cells and γδ T-lymphocytes play a crucial role in the early response to microbial infections. Since both dendritic cells and γδ T-lymphocytes may be activated by specific microbial products, we analyzed their interplay in the presence of such respective ligands: lipopolysaccharide and isopentenyl-pyrophosphate. Activated γδ T-cells increased the maturational state of dendritic cells induced by lipopolysaccharide, increasing the expression of co-stimulatory and MHC class I and II molecules. IL-12 production by dendritic cells was strongly amplified in the presence of activated γδ T-cells and the Th1 polarization of naïve CD4+ T-lymphocytes was significantly increased. On the other hand, dendritic cells enhanced γδ T-cell functions induced by isopentenyl-pyrophosphate and promote their IL-2 independent proliferation through CD86 contact. Altogether, dendritic cells and γδ T-cells exert a complementary function promoting an optimal immune response to non peptidic microbial antigens.
AB - Dendritic cells and γδ T-lymphocytes play a crucial role in the early response to microbial infections. Since both dendritic cells and γδ T-lymphocytes may be activated by specific microbial products, we analyzed their interplay in the presence of such respective ligands: lipopolysaccharide and isopentenyl-pyrophosphate. Activated γδ T-cells increased the maturational state of dendritic cells induced by lipopolysaccharide, increasing the expression of co-stimulatory and MHC class I and II molecules. IL-12 production by dendritic cells was strongly amplified in the presence of activated γδ T-cells and the Th1 polarization of naïve CD4+ T-lymphocytes was significantly increased. On the other hand, dendritic cells enhanced γδ T-cell functions induced by isopentenyl-pyrophosphate and promote their IL-2 independent proliferation through CD86 contact. Altogether, dendritic cells and γδ T-cells exert a complementary function promoting an optimal immune response to non peptidic microbial antigens.
KW - γδ T-lymphocytes
KW - Dendritic cells
KW - Isopentenylpyrophoshate
KW - Lipopolysaccharide
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U2 - 10.1007/s10875-005-4080-8
DO - 10.1007/s10875-005-4080-8
M3 - Article
C2 - 15981088
AN - SCOPUS:21244480514
VL - 25
SP - 230
EP - 237
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
SN - 0271-9142
IS - 3
ER -