Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation

Paul H A Quax, Nina Pedersen, Maria Teresa Masucci, E. Jacoline D Weening-Verhoeff, Keld Danø, Jan H. Verheijen, Francesco Blasi

Research output: Contribution to journalArticle

Abstract

The respective roles of urokinase plasminogen activator (u-PA) and the u-PA receptor in extracellular matrix degradation was investigated. Human prou-PA and the human u-PA receptor were expressed independently by two different mouse LB6 cell lines. The matrix degradation capacity of these cell lines individually or in coculture was studied. Although pro-u-PA-producing cells alone degrade the matrix in the presence of plasminogen, u-PA-receptor producing cells do not. Cocultivation of a small fraction of pro-u-PA-producing cells with the receptor-producing cells increases the rate of matrix degradation at least threefold. By immunoprecipitation it was shown that cocultivation of the two cell lines increases the conversion of the inactive pro-u-PA to the active two chain u-PA. The enhancement of matrix degradation and of pro-u-PA activation requires actual binding of pro-u-PA to its receptor because it is inhibited by u-PA-receptor antagonists. The u-PA receptor must be cell associated, as binding of pro-u-PA to a receptor solubilized from the cell surface with phosphatidylinositol specific phospholipase C did not enhance the activation of pro-u-PA in the presence of plasminogen. The finding that activity of u-PA is enhanced when it is bound to its receptor, even when the receptor is produced by a different cell, might have important implications for the mechanisms of u-PA-induced extracellular proteolysis in vivo.

Original languageEnglish
Pages (from-to)793-803
Number of pages11
JournalMolecular Biology of the Cell
Volume2
Issue number10
Publication statusPublished - Oct 1991

Fingerprint

saruplase
Plasminogen Activators
Urokinase-Type Plasminogen Activator
Extracellular Matrix
Urokinase Plasminogen Activator Receptors
Coculture Techniques
Plasminogen
Cell Line
Phosphoinositide Phospholipase C
Cell Surface Receptors
Immunoprecipitation
Proteolysis

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Quax, P. H. A., Pedersen, N., Masucci, M. T., Weening-Verhoeff, E. J. D., Danø, K., Verheijen, J. H., & Blasi, F. (1991). Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation. Molecular Biology of the Cell, 2(10), 793-803.

Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation. / Quax, Paul H A; Pedersen, Nina; Masucci, Maria Teresa; Weening-Verhoeff, E. Jacoline D; Danø, Keld; Verheijen, Jan H.; Blasi, Francesco.

In: Molecular Biology of the Cell, Vol. 2, No. 10, 10.1991, p. 793-803.

Research output: Contribution to journalArticle

Quax, PHA, Pedersen, N, Masucci, MT, Weening-Verhoeff, EJD, Danø, K, Verheijen, JH & Blasi, F 1991, 'Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation', Molecular Biology of the Cell, vol. 2, no. 10, pp. 793-803.
Quax PHA, Pedersen N, Masucci MT, Weening-Verhoeff EJD, Danø K, Verheijen JH et al. Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation. Molecular Biology of the Cell. 1991 Oct;2(10):793-803.
Quax, Paul H A ; Pedersen, Nina ; Masucci, Maria Teresa ; Weening-Verhoeff, E. Jacoline D ; Danø, Keld ; Verheijen, Jan H. ; Blasi, Francesco. / Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation. In: Molecular Biology of the Cell. 1991 ; Vol. 2, No. 10. pp. 793-803.
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