Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase

Monica Ropolo, Paolo Degan, Mara Foresta, Mariarosaria D'Errico, Denise Lasigliè, Eugenia Dogliotti, Gianluigi Casartelli, Simonetta Zupo, Alessandro Poggi, Guido Frosina

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Repair of the oxidized purine 8-oxo-7,8-dihydroguanine (8-oxoGua) is inefficient in cells belonging to the B complementation group of Cockayne syndrome (CS-B), a developmental and neurological disorder characterized by defective transcription-coupled repair. We show here that cells belonging to the A complementation group (CS-A) are also defective in repair of 8-oxoGua and we demonstrate that expression of the Escherichia coli formamidopyrimidine DNA glycosylase (FPG) completely corrects the repair deficiency in both CS-A and CS-B cells. Phenotypically, CS-A cells are normally sensitive to toxicity and micronuclei induced by the oxidizing agent potassium bromate. CS-B cells display sensitivity to elevated concentrations of potassium bromate but this is not compensated by FPG expression, suggesting toxicity of lesions that are not FPG substrates. The data indicate that 8-oxoGua is not a major toxic and clastogenic lesion in CS cells.

Original languageEnglish
Pages (from-to)1807-1817
Number of pages11
JournalFree Radical Biology and Medicine
Volume42
Issue number12
DOIs
Publication statusPublished - Jun 15 2007

Fingerprint

DNA-Formamidopyrimidine Glycosylase
Cockayne Syndrome
DNA Repair
Escherichia coli
Repair
B-Lymphocytes
Cells
Defects
DNA
Toxicity
Poisons
Transcription
Nervous System Diseases
Oxidants
Substrates
8-hydroxyguanine

Keywords

  • Cockayne syndrome
  • DNA base excision repair
  • Expression
  • Formamidopyrimidine DNA glycosylase
  • Oxidatively damaged DNA

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase. / Ropolo, Monica; Degan, Paolo; Foresta, Mara; D'Errico, Mariarosaria; Lasigliè, Denise; Dogliotti, Eugenia; Casartelli, Gianluigi; Zupo, Simonetta; Poggi, Alessandro; Frosina, Guido.

In: Free Radical Biology and Medicine, Vol. 42, No. 12, 15.06.2007, p. 1807-1817.

Research output: Contribution to journalArticle

Ropolo, Monica ; Degan, Paolo ; Foresta, Mara ; D'Errico, Mariarosaria ; Lasigliè, Denise ; Dogliotti, Eugenia ; Casartelli, Gianluigi ; Zupo, Simonetta ; Poggi, Alessandro ; Frosina, Guido. / Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase. In: Free Radical Biology and Medicine. 2007 ; Vol. 42, No. 12. pp. 1807-1817.
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AU - Lasigliè, Denise

AU - Dogliotti, Eugenia

AU - Casartelli, Gianluigi

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AU - Poggi, Alessandro

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