Complete in vitro prosthesis endothelialization induced by artificial extracellular matrix

P. Pollara, G. Alessandri, S. Bonardelli, A. Simonini, E. Cabibbo, N. Portolani, G. A M Tiberio, S. M. Giulini, A. Turano

Research output: Contribution to journalArticlepeer-review

Abstract

This report presents our research on the conditions necessary to substain optimal in vitro prosthetic endothelialization using human endothelium cultures. Human vein endothelial cells were seeded at a concentration of 3 x 105/cm2 in a gelatinized Dacron patch graft coated with a commercial collagen film, using a solution of fibrin glue. Endothelium adhesion, proliferation, and survival were measured by [3H]thymidine incorporation, after 7 days of incubation. Finally, the morphology of prosthetic endothelialization was analyzed by scanning electron microscopy. We observed that the Dacron patch grafts coated with collagen film were able to promote endothelialization better than the prostheses coated with highly concentrated collagen solution or gelatin. We therefore concluded that the collagen film that supports endothelial cell adhesion and proliferation uniformly covers the entire synthetic endoluminal surface of the Dacron graft, thus preventing endothelial cell alterations induced by direct contact with the synthetic prosthetic surface.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalJournal of Investigative Surgery
Volume12
Issue number2
Publication statusPublished - Mar 1999

Keywords

  • Coated vascular graft
  • Collagen film
  • Endothelial seeding
  • Graft healing
  • Graft prosthesis endothelialization

ASJC Scopus subject areas

  • Surgery

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