Abstract
The brefeldin A ADP-ribosylated substrate, a member of the C-terminal-binding protein family that is referred to as CtBP1/BARS, is a dual-function protein that acts as a transcriptional co-repressor in the nucleus and as an inducer of membrane fission in the cytoplasm. In this review, we first discuss the mechanisms that enable CtBP1/BARS to shift between the nuclear transcriptional co-repressor and the cytosolic fission-inducing activities. Then, we focus on the role of CtBP1/BARS in membrane fission. CtBP1/BARS controls several fission events including macropinocytosis, fluid-phase endocytosis, COPI-coated vesicle formation, basolaterally directed post-Golgi carrier formation, and Golgi partitioning in mitosis. We report on recent advances in our understanding of the CtBP1/BARS membrane fission machineries that operate at the trans-side and at the cis-side of the Golgi complex. Specifically, we discuss how these machineries are assembled and regulated, and how they operate in the formation of the basolaterally directed post-Golgi carriers.
Original language | English |
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Pages (from-to) | 407-421 |
Number of pages | 15 |
Journal | Histochemistry and Cell Biology |
Volume | 140 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- ADP ribosylation
- CtBP1/BARS
- Golgi checkpoint
- Golgi complex
- Membrane fission
- Mitosis
ASJC Scopus subject areas
- Cell Biology
- Histology
- Medical Laboratory Technology
- Molecular Biology