Comprehensive characterization of mesenchymal stromal cells from patients with Fanconi anaemia

Melissa Mantelli, Maria Antonia Avanzini, Vittorio Rosti, Daniela M. Ingo, Antonella Conforti, Francesca Novara, Giulia Arrigo, Marina Boni, Rita Zappatore, Elisa Lenta, Antonia Moretta, Gloria Acquafredda, Annalisa de Silvestri, Valentina Cirillo, Elisa Cicchetti, Mattia Algeri, Luisa Strocchio, Luciana Vinti, Nadia Starc, Simone BiaginiPietro Sirleto, Paolo Bernasconi, Orsetta Zuffardi, Emanuela Maserati, Rita Maccario, Marco Zecca, Franco Locatelli, Maria Ester Bernardo

Research output: Contribution to journalArticle

Abstract

Fanconi anaemia (FA) is an inherited disorder characterized by pancytopenia, congenital malformations and a predisposition to develop malignancies. Alterations in the haematopoietic microenvironment of FA patients have been reported, but little is known regarding the components of their bone marrow (BM) stroma. We characterized mesenchymal stromal cells (MSCs) isolated from BM of 18 FA patients both before and after allogeneic haematopoietic stem cell transplantation (HSCT). Morphology, fibroblast colony-forming unit (CFU-F) ability, proliferative capacity, immunophenotype, differentiation potential, ability to support long-term haematopoiesis and immunomodulatory properties of FA-MSCs were analysed and compared with those of MSCs expanded from 15 age-matched healthy donors (HD-MSCs). FA-MSCs were genetically characterized through conventional karyotyping, diepoxybutane-test and array-comparative genomic hybridization. FA-MSCs generated before and after HSCT were compared. Morphology, immunophenotype, differentiation potential, ability in vitro to inhibit mitogen-induced T-cell proliferation and to support long-term haematopoiesis did not differ between FA-MSCs and HD-MSCs. CFU-F ability and proliferative capacity of FA-MSCs isolated after HSCT were significantly lower than those of HD-MSCs. FA-MSCs reached senescence significantly earlier than HD-MSCs and showed spontaneous chromosome fragility. Our findings indicate that FA-MSCs are defective in their ability to survive in vitro and display spontaneous chromosome breakages; whether these defects are involved in pathophysiology of BM failure syndromes deserves further investigation.

Original languageEnglish
Pages (from-to)826-836
Number of pages11
JournalBritish Journal of Haematology
Volume170
Issue number6
DOIs
Publication statusPublished - Sep 1 2015

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Keywords

  • Bone marrow niche
  • Fanconi anaemia
  • Genetic stability
  • Immunomodulatory properties
  • Mesenchymal stromal cells

ASJC Scopus subject areas

  • Hematology
  • Medicine(all)

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