Computational modeling of combined cell population dynamics and oxygen transport in engineered tissue subject to interstitial perfusion

F. Galbusera, M. Cioffi, M. T. Raimondi, R. Pietrabissa

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

This work presents a computational model of tissue growth under interstitial perfusion inside a tissue engineering bioreactor. The model accounts both for the cell population dynamics, using a model based on cellular automata, and for the hydrodynamic microenvironment imposed by the bioreactor, using a model based on the Lattice-Boltzmann equation and the convection-diffusion equation. The conditions of static culture versus perfused culture were compared, by including the population dynamics along with oxygen diffusion, convective transport and consumption. The model is able to deal with arbitrary complex geometries of the spatial domain; in the present work, the domain modeled was the void space of a porous scaffold for tissue-engineered cartilage. The cell population dynamics algorithm provided results which qualitatively resembled population dynamics patterns observed in experimental studies, and these results were in good quantitative agreement with previous computational studies. Simulation of oxygen transport and consumption showed the fundamental contribution of convective transport in maintaining a high level of oxygen concentration in the whole spatial domain of the scaffold. The model was designed with the aim to be computationally efficient and easily expandable, i.e. to allow straightforward implementability of further models of complex biological phenomena of increasing scientific interest in tissue engineering, such as chemotaxis, extracellular matrix deposition and effect of mechanical stimulation.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalComputer Methods in Biomechanics and Biomedical Engineering
Volume10
Issue number4
DOIs
Publication statusPublished - 2007

Fingerprint

Population dynamics
Population Dynamics
Perfusion
Cells
Tissue
Oxygen
Bioreactors
Tissue Engineering
Tissue Scaffolds
Biological Phenomena
Convection
Tissue engineering
Scaffolds
Chemotaxis
Hydrodynamics
Oxygen Consumption
Cartilage
Extracellular Matrix
Boltzmann equation
Cellular automata

Keywords

  • Cell population dynamics
  • Cellular automaton
  • Lattice Boltzmann
  • Oxygen distribution

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Computer Science Applications
  • Human-Computer Interaction
  • Medicine(all)

Cite this

Computational modeling of combined cell population dynamics and oxygen transport in engineered tissue subject to interstitial perfusion. / Galbusera, F.; Cioffi, M.; Raimondi, M. T.; Pietrabissa, R.

In: Computer Methods in Biomechanics and Biomedical Engineering, Vol. 10, No. 4, 2007, p. 279-287.

Research output: Contribution to journalArticle

@article{9eaaeea2ad734210a70ee8c82e2a3b4b,
title = "Computational modeling of combined cell population dynamics and oxygen transport in engineered tissue subject to interstitial perfusion",
abstract = "This work presents a computational model of tissue growth under interstitial perfusion inside a tissue engineering bioreactor. The model accounts both for the cell population dynamics, using a model based on cellular automata, and for the hydrodynamic microenvironment imposed by the bioreactor, using a model based on the Lattice-Boltzmann equation and the convection-diffusion equation. The conditions of static culture versus perfused culture were compared, by including the population dynamics along with oxygen diffusion, convective transport and consumption. The model is able to deal with arbitrary complex geometries of the spatial domain; in the present work, the domain modeled was the void space of a porous scaffold for tissue-engineered cartilage. The cell population dynamics algorithm provided results which qualitatively resembled population dynamics patterns observed in experimental studies, and these results were in good quantitative agreement with previous computational studies. Simulation of oxygen transport and consumption showed the fundamental contribution of convective transport in maintaining a high level of oxygen concentration in the whole spatial domain of the scaffold. The model was designed with the aim to be computationally efficient and easily expandable, i.e. to allow straightforward implementability of further models of complex biological phenomena of increasing scientific interest in tissue engineering, such as chemotaxis, extracellular matrix deposition and effect of mechanical stimulation.",
keywords = "Cell population dynamics, Cellular automaton, Lattice Boltzmann, Oxygen distribution",
author = "F. Galbusera and M. Cioffi and Raimondi, {M. T.} and R. Pietrabissa",
year = "2007",
doi = "10.1080/10255840701318404",
language = "English",
volume = "10",
pages = "279--287",
journal = "Computer Methods in Biomechanics and Biomedical Engineering",
issn = "1025-5842",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Computational modeling of combined cell population dynamics and oxygen transport in engineered tissue subject to interstitial perfusion

AU - Galbusera, F.

AU - Cioffi, M.

AU - Raimondi, M. T.

AU - Pietrabissa, R.

PY - 2007

Y1 - 2007

N2 - This work presents a computational model of tissue growth under interstitial perfusion inside a tissue engineering bioreactor. The model accounts both for the cell population dynamics, using a model based on cellular automata, and for the hydrodynamic microenvironment imposed by the bioreactor, using a model based on the Lattice-Boltzmann equation and the convection-diffusion equation. The conditions of static culture versus perfused culture were compared, by including the population dynamics along with oxygen diffusion, convective transport and consumption. The model is able to deal with arbitrary complex geometries of the spatial domain; in the present work, the domain modeled was the void space of a porous scaffold for tissue-engineered cartilage. The cell population dynamics algorithm provided results which qualitatively resembled population dynamics patterns observed in experimental studies, and these results were in good quantitative agreement with previous computational studies. Simulation of oxygen transport and consumption showed the fundamental contribution of convective transport in maintaining a high level of oxygen concentration in the whole spatial domain of the scaffold. The model was designed with the aim to be computationally efficient and easily expandable, i.e. to allow straightforward implementability of further models of complex biological phenomena of increasing scientific interest in tissue engineering, such as chemotaxis, extracellular matrix deposition and effect of mechanical stimulation.

AB - This work presents a computational model of tissue growth under interstitial perfusion inside a tissue engineering bioreactor. The model accounts both for the cell population dynamics, using a model based on cellular automata, and for the hydrodynamic microenvironment imposed by the bioreactor, using a model based on the Lattice-Boltzmann equation and the convection-diffusion equation. The conditions of static culture versus perfused culture were compared, by including the population dynamics along with oxygen diffusion, convective transport and consumption. The model is able to deal with arbitrary complex geometries of the spatial domain; in the present work, the domain modeled was the void space of a porous scaffold for tissue-engineered cartilage. The cell population dynamics algorithm provided results which qualitatively resembled population dynamics patterns observed in experimental studies, and these results were in good quantitative agreement with previous computational studies. Simulation of oxygen transport and consumption showed the fundamental contribution of convective transport in maintaining a high level of oxygen concentration in the whole spatial domain of the scaffold. The model was designed with the aim to be computationally efficient and easily expandable, i.e. to allow straightforward implementability of further models of complex biological phenomena of increasing scientific interest in tissue engineering, such as chemotaxis, extracellular matrix deposition and effect of mechanical stimulation.

KW - Cell population dynamics

KW - Cellular automaton

KW - Lattice Boltzmann

KW - Oxygen distribution

UR - http://www.scopus.com/inward/record.url?scp=34848881862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34848881862&partnerID=8YFLogxK

U2 - 10.1080/10255840701318404

DO - 10.1080/10255840701318404

M3 - Article

VL - 10

SP - 279

EP - 287

JO - Computer Methods in Biomechanics and Biomedical Engineering

JF - Computer Methods in Biomechanics and Biomedical Engineering

SN - 1025-5842

IS - 4

ER -