Computational reconstruction of pacemaking and intrinsic electroresponsiveness in cerebellar golgi cells

Sergio Solinas, Lia Forti, Elisabetta Cesana, Jonathan Mapelli, Erik De Schutter, Egidio D'Angelo

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The Golgi cells have been recently shown to beat regularly in vitro (Forti et al., 2006. J. Physiol. 574, 711-729). Four main currents were shown to be involved, namely a persistent sodium current (INa-p), an h current (Ih), an SK-type calcium-dependent potassium current (IK-AHP), and a slow M-like potassium current (IK-slow). These ionic currents could take part, together with others, also to different aspects of neuronal excitability like responses to depolarizing and hyperpolarizing current injection. However, the ionic mechanisms and their interactions remained largely hypothetical. In this work, we have investigated the mechanisms of Golgi cell excitability by developing a computational model. The model predicts that pacemaking is sustained by subthreshold oscillations tightly coupled to spikes. INa-p and IK-slow emerged as the critical determinants of oscillations. Ih also played a role by setting the oscillatory mechanism into the appropriate membrane potential range. IK-AHP, though taking part to the oscillation, appeared primarily involved in regulating the ISI following spikes. The combination with other currents, in particular a resurgent sodium current (INa-r) and an A-current (IK-A), allowed a precise regulation of response frequency and delay. These results provide a coherent reconstruction of the ionic mechanisms determining Golgi cell intrinsic electroresponsiveness and suggests important implications for cerebellar signal processing, which will be fully developed in a companion paper.

Original languageEnglish
Article number2
JournalFrontiers in Cellular Neuroscience
Volume1
Issue numberDEC
DOIs
Publication statusPublished - Dec 30 2007

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Sodium
Potassium
Membrane Potentials
Calcium
Injections
In Vitro Techniques

Keywords

  • Cerebellum
  • Golgi cell
  • Granular layer
  • Modeling
  • Pacemaking

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Computational reconstruction of pacemaking and intrinsic electroresponsiveness in cerebellar golgi cells. / Solinas, Sergio; Forti, Lia; Cesana, Elisabetta; Mapelli, Jonathan; De Schutter, Erik; D'Angelo, Egidio.

In: Frontiers in Cellular Neuroscience, Vol. 1, No. DEC, 2, 30.12.2007.

Research output: Contribution to journalArticle

Solinas, Sergio ; Forti, Lia ; Cesana, Elisabetta ; Mapelli, Jonathan ; De Schutter, Erik ; D'Angelo, Egidio. / Computational reconstruction of pacemaking and intrinsic electroresponsiveness in cerebellar golgi cells. In: Frontiers in Cellular Neuroscience. 2007 ; Vol. 1, No. DEC.
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