Abstract
Treatment of asthma with corticosteroids results in downregulation of eosinophilic airway inflammation. We evaluated in vitro the activity of an "inhaled" corticosteroid, mometasone furoate (MF), and of a "systemic" corticosteroid, dexamethasone (DEX), on eosinophil functions, i.e. adhesion molecule expression and cell chemotaxis. Partially purified blood eosinophils were obtained from 18 asthmatic subjects sensitized to house dust mites. The expression of the macrophage antigen (Mac)-1 (CD11b/CD18) was measured by specific monoclonal antibody (mAb) staining and flow cytometry analysis at baseline or after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or with recombinant human (rh) granulocyte macrophage-colony stimulating factor (GM-CSF) plus a mAb anti-human (ah) IgE low affinity receptor [FcεRII or CD23]. Cell chemotaxis toward the complement fragment 5a (C5a) or rh interleukin (IL)-5 was evaluated in Boyden microchambers by light microscopy. Eosinophils showed a significant increase in Mac-1 expression after activation with fMLP or with rh GM-CSF plus ah CD23 mAbs (p
| Original language | English |
|---|---|
| Pages (from-to) | 1687-1696 |
| Number of pages | 10 |
| Journal | International Immunopharmacology |
| Volume | 4 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 2004 |
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Keywords
- Chemotaxis
- Corticosteroids
- Eosinophils
- Mac-1 expression
ASJC Scopus subject areas
- Immunology
- Pharmacology
Cite this
Concentration-dependent activity of mometasone furoate and dexamethasone on blood eosinophils isolated from atopic children : Modulation of Mac-1 expression and chemotaxis. / Sale, Rosa; Sabatini, Federica; Silvestri, Michela; Serpero, Laura; Petecchia, Loredana; Rossi, Giovanni A.
In: International Immunopharmacology, Vol. 4, No. 13, 2004, p. 1687-1696.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Concentration-dependent activity of mometasone furoate and dexamethasone on blood eosinophils isolated from atopic children
T2 - Modulation of Mac-1 expression and chemotaxis
AU - Sale, Rosa
AU - Sabatini, Federica
AU - Silvestri, Michela
AU - Serpero, Laura
AU - Petecchia, Loredana
AU - Rossi, Giovanni A.
PY - 2004
Y1 - 2004
N2 - Treatment of asthma with corticosteroids results in downregulation of eosinophilic airway inflammation. We evaluated in vitro the activity of an "inhaled" corticosteroid, mometasone furoate (MF), and of a "systemic" corticosteroid, dexamethasone (DEX), on eosinophil functions, i.e. adhesion molecule expression and cell chemotaxis. Partially purified blood eosinophils were obtained from 18 asthmatic subjects sensitized to house dust mites. The expression of the macrophage antigen (Mac)-1 (CD11b/CD18) was measured by specific monoclonal antibody (mAb) staining and flow cytometry analysis at baseline or after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or with recombinant human (rh) granulocyte macrophage-colony stimulating factor (GM-CSF) plus a mAb anti-human (ah) IgE low affinity receptor [FcεRII or CD23]. Cell chemotaxis toward the complement fragment 5a (C5a) or rh interleukin (IL)-5 was evaluated in Boyden microchambers by light microscopy. Eosinophils showed a significant increase in Mac-1 expression after activation with fMLP or with rh GM-CSF plus ah CD23 mAbs (p
AB - Treatment of asthma with corticosteroids results in downregulation of eosinophilic airway inflammation. We evaluated in vitro the activity of an "inhaled" corticosteroid, mometasone furoate (MF), and of a "systemic" corticosteroid, dexamethasone (DEX), on eosinophil functions, i.e. adhesion molecule expression and cell chemotaxis. Partially purified blood eosinophils were obtained from 18 asthmatic subjects sensitized to house dust mites. The expression of the macrophage antigen (Mac)-1 (CD11b/CD18) was measured by specific monoclonal antibody (mAb) staining and flow cytometry analysis at baseline or after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or with recombinant human (rh) granulocyte macrophage-colony stimulating factor (GM-CSF) plus a mAb anti-human (ah) IgE low affinity receptor [FcεRII or CD23]. Cell chemotaxis toward the complement fragment 5a (C5a) or rh interleukin (IL)-5 was evaluated in Boyden microchambers by light microscopy. Eosinophils showed a significant increase in Mac-1 expression after activation with fMLP or with rh GM-CSF plus ah CD23 mAbs (p
KW - Chemotaxis
KW - Corticosteroids
KW - Eosinophils
KW - Mac-1 expression
UR - http://www.scopus.com/inward/record.url?scp=5344230713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5344230713&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2004.07.029
DO - 10.1016/j.intimp.2004.07.029
M3 - Article
C2 - 15454120
AN - SCOPUS:5344230713
VL - 4
SP - 1687
EP - 1696
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 13
ER -