Concentration-dependent activity of mometasone furoate and dexamethasone on blood eosinophils isolated from atopic children: Modulation of Mac-1 expression and chemotaxis

Rosa Sale, Federica Sabatini, Michela Silvestri, Laura Serpero, Loredana Petecchia, Giovanni A. Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

Treatment of asthma with corticosteroids results in downregulation of eosinophilic airway inflammation. We evaluated in vitro the activity of an "inhaled" corticosteroid, mometasone furoate (MF), and of a "systemic" corticosteroid, dexamethasone (DEX), on eosinophil functions, i.e. adhesion molecule expression and cell chemotaxis. Partially purified blood eosinophils were obtained from 18 asthmatic subjects sensitized to house dust mites. The expression of the macrophage antigen (Mac)-1 (CD11b/CD18) was measured by specific monoclonal antibody (mAb) staining and flow cytometry analysis at baseline or after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or with recombinant human (rh) granulocyte macrophage-colony stimulating factor (GM-CSF) plus a mAb anti-human (ah) IgE low affinity receptor [FcεRII or CD23]. Cell chemotaxis toward the complement fragment 5a (C5a) or rh interleukin (IL)-5 was evaluated in Boyden microchambers by light microscopy. Eosinophils showed a significant increase in Mac-1 expression after activation with fMLP or with rh GM-CSF plus ah CD23 mAbs (p

Original languageEnglish
Pages (from-to)1687-1696
Number of pages10
JournalInternational Immunopharmacology
Volume4
Issue number13
DOIs
Publication statusPublished - 2004

Keywords

  • Chemotaxis
  • Corticosteroids
  • Eosinophils
  • Mac-1 expression

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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