Concentration of human hematopoietic stem cells in bone marrow transplantation: Results of a multicenter study using Baxter CS 3000 plus cell separator

A. Angelini, P. Accorsi, A. Iacone, T. Bonfini, C. Refe, A. Olivieri, U. Bodini, C. Bergonzi, E. Incarbone, G. Adorno, F. Bertola, G. Fattori, G. Torlontano

Research output: Contribution to journalArticle

Abstract

Preliminary BM processing to produce an enriched MNC fraction from large BM volumes improves subsequent pharmacological and/or immunological 'ex vivo' treatment and cryopreservation. We detail on a multicenter study (6 Transplant Centers) performed to establish an effective and reliable protocol using a CS 3000 continuous flow separator on a large series of BM processed for autologous (96) and allogeneic (12) transplantation. The reduction in volume was 78.6+7.2% while 28.9+12.4% of the original nucleated cells were found in the final product. A mean of 84.3+13.2% of the starting MNC was yielded in a fraction containing over 81% MNC. Cloning efficiency indicated than the final graft was highly enriched in progenitor cells committed to the granulocyte/macrophage pathway (>100%) as assessed in vitro (CFU-GM). Removal of RBC and PLT was 98.3+1.1 and 37.7+14.6%, respectively. The mean dose of MNC and CFU-GM was 0.6+0.37 x 106 and 0.96+1 x 105 recipient weight. The entire process was accomplished in 87.5+20 min. We concluded that this automated device is a simple and reproducible method for BM processing suitable as first step for further 'ex vivo' automated negative and/or positive cell selections.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalInternational Journal of Artificial Organs
Volume16
Issue numberSUPPL. 5
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Concentration of human hematopoietic stem cells in bone marrow transplantation: Results of a multicenter study using Baxter CS 3000 plus cell separator'. Together they form a unique fingerprint.

  • Cite this

    Angelini, A., Accorsi, P., Iacone, A., Bonfini, T., Refe, C., Olivieri, A., Bodini, U., Bergonzi, C., Incarbone, E., Adorno, G., Bertola, F., Fattori, G., & Torlontano, G. (1993). Concentration of human hematopoietic stem cells in bone marrow transplantation: Results of a multicenter study using Baxter CS 3000 plus cell separator. International Journal of Artificial Organs, 16(SUPPL. 5), 13-18.