Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms

A. Minelli, E. Capra, C. Dellavecchia, C. Danesino, C. Olivieri, S. Tateo, P. Tenti, S. Scappaticci

Research output: Contribution to journalArticle

Abstract

We studied a goup of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) were split in two parts, one was used for short term cultures for cytogenetic investigation while from the second DNA was extracted for molecular studies. We studied 22 polymorphic loci from 19 chromosomes and compared the alleles observed in the tumor with those observed in the DNA obtained from peripheral blood. Extensive loss of heterozigosity was observed when total or partial chromosomal loss was observed in at least 50% of the examined cells; MIN did not correlate with any particular cytogenetic abnormality nor with LOH.

Original languageEnglish
Pages (from-to)234-238
Number of pages5
JournalEuropean Journal of Gynaecological Oncology
Volume19
Issue number3
Publication statusPublished - 1998

Fingerprint

Microsatellite Instability
Uterine Neoplasms
Cytogenetics
Chromosome Aberrations
Ovarian Neoplasms
Chromosomes, Human, Pair 19
DNA
Neoplasms
Alleles

Keywords

  • Chromosome abnormalities
  • Loss of heterozygosity (LOH)
  • Microsatellite instability (MI)
  • Uterine and ovarian cancers

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Minelli, A., Capra, E., Dellavecchia, C., Danesino, C., Olivieri, C., Tateo, S., ... Scappaticci, S. (1998). Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms. European Journal of Gynaecological Oncology, 19(3), 234-238.

Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms. / Minelli, A.; Capra, E.; Dellavecchia, C.; Danesino, C.; Olivieri, C.; Tateo, S.; Tenti, P.; Scappaticci, S.

In: European Journal of Gynaecological Oncology, Vol. 19, No. 3, 1998, p. 234-238.

Research output: Contribution to journalArticle

Minelli, A, Capra, E, Dellavecchia, C, Danesino, C, Olivieri, C, Tateo, S, Tenti, P & Scappaticci, S 1998, 'Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms', European Journal of Gynaecological Oncology, vol. 19, no. 3, pp. 234-238.
Minelli, A. ; Capra, E. ; Dellavecchia, C. ; Danesino, C. ; Olivieri, C. ; Tateo, S. ; Tenti, P. ; Scappaticci, S. / Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms. In: European Journal of Gynaecological Oncology. 1998 ; Vol. 19, No. 3. pp. 234-238.
@article{167c5e814f9f4c9d93e26328f8d6a8d8,
title = "Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms",
abstract = "We studied a goup of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) were split in two parts, one was used for short term cultures for cytogenetic investigation while from the second DNA was extracted for molecular studies. We studied 22 polymorphic loci from 19 chromosomes and compared the alleles observed in the tumor with those observed in the DNA obtained from peripheral blood. Extensive loss of heterozigosity was observed when total or partial chromosomal loss was observed in at least 50{\%} of the examined cells; MIN did not correlate with any particular cytogenetic abnormality nor with LOH.",
keywords = "Chromosome abnormalities, Loss of heterozygosity (LOH), Microsatellite instability (MI), Uterine and ovarian cancers",
author = "A. Minelli and E. Capra and C. Dellavecchia and C. Danesino and C. Olivieri and S. Tateo and P. Tenti and S. Scappaticci",
year = "1998",
language = "English",
volume = "19",
pages = "234--238",
journal = "European Journal of Gynaecological Oncology",
issn = "0392-2936",
publisher = "S.O.G. CANADA Inc.",
number = "3",

}

TY - JOUR

T1 - Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms

AU - Minelli, A.

AU - Capra, E.

AU - Dellavecchia, C.

AU - Danesino, C.

AU - Olivieri, C.

AU - Tateo, S.

AU - Tenti, P.

AU - Scappaticci, S.

PY - 1998

Y1 - 1998

N2 - We studied a goup of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) were split in two parts, one was used for short term cultures for cytogenetic investigation while from the second DNA was extracted for molecular studies. We studied 22 polymorphic loci from 19 chromosomes and compared the alleles observed in the tumor with those observed in the DNA obtained from peripheral blood. Extensive loss of heterozigosity was observed when total or partial chromosomal loss was observed in at least 50% of the examined cells; MIN did not correlate with any particular cytogenetic abnormality nor with LOH.

AB - We studied a goup of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) were split in two parts, one was used for short term cultures for cytogenetic investigation while from the second DNA was extracted for molecular studies. We studied 22 polymorphic loci from 19 chromosomes and compared the alleles observed in the tumor with those observed in the DNA obtained from peripheral blood. Extensive loss of heterozigosity was observed when total or partial chromosomal loss was observed in at least 50% of the examined cells; MIN did not correlate with any particular cytogenetic abnormality nor with LOH.

KW - Chromosome abnormalities

KW - Loss of heterozygosity (LOH)

KW - Microsatellite instability (MI)

KW - Uterine and ovarian cancers

UR - http://www.scopus.com/inward/record.url?scp=0031896492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031896492&partnerID=8YFLogxK

M3 - Article

C2 - 9641220

AN - SCOPUS:0031896492

VL - 19

SP - 234

EP - 238

JO - European Journal of Gynaecological Oncology

JF - European Journal of Gynaecological Oncology

SN - 0392-2936

IS - 3

ER -