Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis

Patrizia Danieli, Giuseppe Malpasso, Maria Chiara Ciuffreda, Elisabetta Cervio, Laura Calvillo, Francesco Copes, Federica Pisano, Manuela Mura, Lennaert Kleijn, Rudolf A. De Boer, Gianluca Viarengo, Vittorio Rosti, Arsenio Spinillo, Marianna Roccio, Massimiliano Gnecchi

Research output: Contribution to journalArticle

Abstract

The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. The goal of the present study was to elucidate whether hAMCs can exert beneficial paracrine effects on infarcted rat hearts, in particular through cardio-protection and angiogenesis. Moreover, we aimed to identify the putative active paracrine medi-ators.hAMCswereisolated,expanded,andcharacterized.Invitro,conditionedmediumfromhAMC (hAMC-CM) exhibited cytoprotective and proangiogenic properties. In vivo, injection of hAMC-CM into infarcted rat hearts limited the infarct size, reduced cardiomyocyte apoptosis and ventricular remodeling, andstrongly promoted capillary formation at theinfarct border zone. Genearray analysis led to the identification of 32 genes encoding for the secreted factors overexpressed by hAMCs. Among these, midkine and secreted protein acidic and rich in cysteine were also upregulated at the protein level. Furthermore, high amounts of several proangiogenic factorswere detected in hAMC-CMbycytokine array.Ourresultsstronglysupport theconcept thattheadministrationof hAMC-CM favors the repair process after acute myocardial infarction.

Original languageEnglish
Pages (from-to)448-458
Number of pages11
JournalStem cells translational medicine
Volume4
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • Cardiac
  • Clinical translation
  • Fetal stem cells
  • Placenta

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

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