Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis

Patrizia Danieli, Giuseppe Malpasso, Maria Chiara Ciuffreda, Elisabetta Cervio, Laura Calvillo, Francesco Copes, Federica Pisano, Manuela Mura, Lennaert Kleijn, Rudolf A. De Boer, Gianluca Viarengo, Vittorio Rosti, Arsenio Spinillo, Marianna Roccio, Massimiliano Gnecchi

Research output: Contribution to journalArticle

Abstract

The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. The goal of the present study was to elucidate whether hAMCs can exert beneficial paracrine effects on infarcted rat hearts, in particular through cardio-protection and angiogenesis. Moreover, we aimed to identify the putative active paracrine medi-ators.hAMCswereisolated,expanded,andcharacterized.Invitro,conditionedmediumfromhAMC (hAMC-CM) exhibited cytoprotective and proangiogenic properties. In vivo, injection of hAMC-CM into infarcted rat hearts limited the infarct size, reduced cardiomyocyte apoptosis and ventricular remodeling, andstrongly promoted capillary formation at theinfarct border zone. Genearray analysis led to the identification of 32 genes encoding for the secreted factors overexpressed by hAMCs. Among these, midkine and secreted protein acidic and rich in cysteine were also upregulated at the protein level. Furthermore, high amounts of several proangiogenic factorswere detected in hAMC-CMbycytokine array.Ourresultsstronglysupport theconcept thattheadministrationof hAMC-CM favors the repair process after acute myocardial infarction.

Original languageEnglish
Pages (from-to)448-458
Number of pages11
JournalStem cells translational medicine
Volume4
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Amnion
Conditioned Culture Medium
Mesenchymal Stromal Cells
Ventricular Remodeling
Cardiac Myocytes
Cysteine
Proteins
Myocardial Infarction
Apoptosis
Injections
Genes

Keywords

  • Cardiac
  • Clinical translation
  • Fetal stem cells
  • Placenta

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

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title = "Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis",
abstract = "The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. The goal of the present study was to elucidate whether hAMCs can exert beneficial paracrine effects on infarcted rat hearts, in particular through cardio-protection and angiogenesis. Moreover, we aimed to identify the putative active paracrine medi-ators.hAMCswereisolated,expanded,andcharacterized.Invitro,conditionedmediumfromhAMC (hAMC-CM) exhibited cytoprotective and proangiogenic properties. In vivo, injection of hAMC-CM into infarcted rat hearts limited the infarct size, reduced cardiomyocyte apoptosis and ventricular remodeling, andstrongly promoted capillary formation at theinfarct border zone. Genearray analysis led to the identification of 32 genes encoding for the secreted factors overexpressed by hAMCs. Among these, midkine and secreted protein acidic and rich in cysteine were also upregulated at the protein level. Furthermore, high amounts of several proangiogenic factorswere detected in hAMC-CMbycytokine array.Ourresultsstronglysupport theconcept thattheadministrationof hAMC-CM favors the repair process after acute myocardial infarction.",
keywords = "Cardiac, Clinical translation, Fetal stem cells, Placenta",
author = "Patrizia Danieli and Giuseppe Malpasso and Ciuffreda, {Maria Chiara} and Elisabetta Cervio and Laura Calvillo and Francesco Copes and Federica Pisano and Manuela Mura and Lennaert Kleijn and {De Boer}, {Rudolf A.} and Gianluca Viarengo and Vittorio Rosti and Arsenio Spinillo and Marianna Roccio and Massimiliano Gnecchi",
year = "2015",
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pages = "448--458",
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TY - JOUR

T1 - Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis

AU - Danieli, Patrizia

AU - Malpasso, Giuseppe

AU - Ciuffreda, Maria Chiara

AU - Cervio, Elisabetta

AU - Calvillo, Laura

AU - Copes, Francesco

AU - Pisano, Federica

AU - Mura, Manuela

AU - Kleijn, Lennaert

AU - De Boer, Rudolf A.

AU - Viarengo, Gianluca

AU - Rosti, Vittorio

AU - Spinillo, Arsenio

AU - Roccio, Marianna

AU - Gnecchi, Massimiliano

PY - 2015

Y1 - 2015

N2 - The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. The goal of the present study was to elucidate whether hAMCs can exert beneficial paracrine effects on infarcted rat hearts, in particular through cardio-protection and angiogenesis. Moreover, we aimed to identify the putative active paracrine medi-ators.hAMCswereisolated,expanded,andcharacterized.Invitro,conditionedmediumfromhAMC (hAMC-CM) exhibited cytoprotective and proangiogenic properties. In vivo, injection of hAMC-CM into infarcted rat hearts limited the infarct size, reduced cardiomyocyte apoptosis and ventricular remodeling, andstrongly promoted capillary formation at theinfarct border zone. Genearray analysis led to the identification of 32 genes encoding for the secreted factors overexpressed by hAMCs. Among these, midkine and secreted protein acidic and rich in cysteine were also upregulated at the protein level. Furthermore, high amounts of several proangiogenic factorswere detected in hAMC-CMbycytokine array.Ourresultsstronglysupport theconcept thattheadministrationof hAMC-CM favors the repair process after acute myocardial infarction.

AB - The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. The goal of the present study was to elucidate whether hAMCs can exert beneficial paracrine effects on infarcted rat hearts, in particular through cardio-protection and angiogenesis. Moreover, we aimed to identify the putative active paracrine medi-ators.hAMCswereisolated,expanded,andcharacterized.Invitro,conditionedmediumfromhAMC (hAMC-CM) exhibited cytoprotective and proangiogenic properties. In vivo, injection of hAMC-CM into infarcted rat hearts limited the infarct size, reduced cardiomyocyte apoptosis and ventricular remodeling, andstrongly promoted capillary formation at theinfarct border zone. Genearray analysis led to the identification of 32 genes encoding for the secreted factors overexpressed by hAMCs. Among these, midkine and secreted protein acidic and rich in cysteine were also upregulated at the protein level. Furthermore, high amounts of several proangiogenic factorswere detected in hAMC-CMbycytokine array.Ourresultsstronglysupport theconcept thattheadministrationof hAMC-CM favors the repair process after acute myocardial infarction.

KW - Cardiac

KW - Clinical translation

KW - Fetal stem cells

KW - Placenta

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