Conditions of endoplasmic reticulum stress favor the accumulation of cytosolic prion protein

Andrea Orsi, Luana Fioriti, Roberto Chiesa, Roberto Sitia

Research output: Contribution to journalArticle

Abstract

After signal sequence-dependent targeting to the endoplasmic reticulum (ER), prion protein (PrP) undergoes several post-translational modifications, including glycosylation, disulfide bond formation, and the addition of a glycosylphosphatidylinositol anchor. As a result, multiple isoforms are generated. Because of the intrinsic weakness of the PrP signal sequence, a fraction of newly synthesized molecules fails to translocate and localizes to the cytosol. The physiopathologic role of this cytosolic isoform is still being debated. Here we have shown that, in both cultured cell lines and primary neurons, ER stress conditions weaken PrP co-translational translocation, favoring accumulation of aggregation-prone cytosolic species, which retain the signal sequence but lack N-glycans and disulfides. Inhibition of proteasomes further increases the levels of cytosolic PrP. Overexpression of spliced XBP1 facilitates ER translocation, suggesting that downstream elements of the Ire1-XBP1 pathway are involved in PrP targeting. These studies reveal a link between ER stress and the formation of cytosolic PrP isoforms potentially endowed with novel signaling or cytotoxic functions.

Original languageEnglish
Pages (from-to)30431-30438
Number of pages8
JournalJournal of Biological Chemistry
Volume281
Issue number41
DOIs
Publication statusPublished - Oct 13 2006

ASJC Scopus subject areas

  • Biochemistry

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