Confocal laser scanning microscopy to study formation and properties of polyelectrolyte nanocapsules derived from CdCO3 templates

D. Silvano, S. Krol, A. Diaspro, O. Cavalleri, A. Gliozzi

Research output: Contribution to journalArticlepeer-review

Abstract

Three-dimensional confocal laser scanning microscopy (CLSM) was used as an essential investigation method to obtain information about the formation and morphological characteristics of nanocapsules. Nanocapsules are built by layer-by-layer deposition of alternatively charged polyelectrolytes on templates forming nanostructured hollow shells. CLSM is unique in allowing for monitoring of the core dissolution process in real time and for studying nanocapsule functioning in hydrated conditions within a three-dimensional and temporal framework. Since we are also interested in the identification of other possible templates, we briefly report on the use of yeast cells as biocolloidal cores monitored by means of two-photon microscopy. Here we focus our attention on the use of CdCO3 crystals as template candidates for the preparation of stable capsules. Both cubic and spherical CdCO3 cores have been produced. Cubic cores exhibit higher monodispersity and smaller size compared to spherical ones. Capsules templated on these cores have a higher surface-to-volume ratio that is valuable for applications related to drug delivery, functional properties of the shells and adsorption of proteins, and other biologically relevant molecules.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalMicroscopy Research and Technique
Volume59
Issue number6
DOIs
Publication statusPublished - Dec 15 2002

Keywords

  • Biocolloids
  • Confocal laser scanning microscopy
  • Crystal templates
  • Nanocapsules
  • Polyelectrolyte
  • Two-photon excitation microscopy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

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