Confocal microscopy for high-resolution and high-content analysis of the cell cycle

Research output: Contribution to journalReview articlepeer-review

Abstract

Optical fluorescence microscopy offers a wide range of technological solutions to address many questions in biomedical research. Spatial resolution has been greatly improved by the use of confocal microscopes, providing a 3-D analysis of the intracellular space. Automation has contributed to make confocal analysis available for high-content image cytometry studies. However, the storage, browsing, and analysis of the amount of data generated can challenge the feasibility of such studies. Presented in this chapter is a multistep acquisition and analysis protocol that can bypass such difficulties by an analysis-driven data collection. Cell-cycle analysis of low-resolution data can be employed to select cell populations of interest that can then be imaged at extremely high resolution and subjected to high-content analysis.

Original languageEnglish
Pages (from-to)7.42.1-7.42.14
JournalCurrent Protocols in Cytometry
Volume70
DOIs
Publication statusPublished - 2014

Keywords

  • automated microscopy
  • cell cycle
  • confocal microscopy
  • high content
  • high resolution
  • image cytometry
  • S phase

ASJC Scopus subject areas

  • Medicine(all)

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