Abstract
Optical fluorescence microscopy offers a wide range of technological solutions to address many questions in biomedical research. Spatial resolution has been greatly improved by the use of confocal microscopes, providing a 3-D analysis of the intracellular space. Automation has contributed to make confocal analysis available for high-content image cytometry studies. However, the storage, browsing, and analysis of the amount of data generated can challenge the feasibility of such studies. Presented in this chapter is a multistep acquisition and analysis protocol that can bypass such difficulties by an analysis-driven data collection. Cell-cycle analysis of low-resolution data can be employed to select cell populations of interest that can then be imaged at extremely high resolution and subjected to high-content analysis.
Original language | English |
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Pages (from-to) | 7.42.1-7.42.14 |
Journal | Current Protocols in Cytometry |
Volume | 70 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- automated microscopy
- cell cycle
- confocal microscopy
- high content
- high resolution
- image cytometry
- S phase
ASJC Scopus subject areas
- Medicine(all)