TY - JOUR
T1 - Conformal coating by multilayer nano-encapsulation for the protection of human pancreatic islets
T2 - In-vitro and in-vivo studies
AU - Syed, Farooq
AU - Bugliani, Marco
AU - Novelli, Michela
AU - Olimpico, Francesco
AU - Suleiman, Mara
AU - Marselli, Lorella
AU - Boggi, Ugo
AU - Filipponi, Franco
AU - Raffa, Vittoria
AU - Krol, Silke
AU - Campani, Daniela
AU - Masiello, Pellegrino
AU - De Tata, Vincenzo
AU - Marchetti, Piero
PY - 2018/10/1
Y1 - 2018/10/1
N2 - To improve the efficiency of pancreatic islet transplantation, we performed in-vitro and in-vivo experiments with isolated human pancreatic islets coated by multi-layer nano-encapsulation using differently charged polymers [chitosan and poly(sodium styrene sulfonate)] to obtain up to 9 layers. The islet coating (thickness: 104.2 ± 4.2 nm) was uniform, with ≥ 90% cell viability and well preserved beta- and alpha-cell ultrastructure. Nano-encapsulated islets maintained physiological glucose-stimulated insulin secretion by both static incubation and perifusion studies. Notably, palmitate- or cytokine-induced toxicity was significantly reduced in nano-coated islets. Xenotransplantation of nano-encapsulated islets under the kidney capsule of streptozotocin-induced C57Bl/6J diabetic mice allowed long term normal or near normal glycemia, associated with minimal infiltration of immune cell into the grafts, well preserved islet morphology and signs of re-vascularization. In summary, the multi-layer nano-encapsulation approach described in the present study provides a promising tool to effectively protect human islets both in-vitro and in-vivo conditions.
AB - To improve the efficiency of pancreatic islet transplantation, we performed in-vitro and in-vivo experiments with isolated human pancreatic islets coated by multi-layer nano-encapsulation using differently charged polymers [chitosan and poly(sodium styrene sulfonate)] to obtain up to 9 layers. The islet coating (thickness: 104.2 ± 4.2 nm) was uniform, with ≥ 90% cell viability and well preserved beta- and alpha-cell ultrastructure. Nano-encapsulated islets maintained physiological glucose-stimulated insulin secretion by both static incubation and perifusion studies. Notably, palmitate- or cytokine-induced toxicity was significantly reduced in nano-coated islets. Xenotransplantation of nano-encapsulated islets under the kidney capsule of streptozotocin-induced C57Bl/6J diabetic mice allowed long term normal or near normal glycemia, associated with minimal infiltration of immune cell into the grafts, well preserved islet morphology and signs of re-vascularization. In summary, the multi-layer nano-encapsulation approach described in the present study provides a promising tool to effectively protect human islets both in-vitro and in-vivo conditions.
KW - Diabetes
KW - Human islets
KW - Immune isolation
KW - Islets transplantation
KW - Multilayer nanoencapsulation
UR - http://www.scopus.com/inward/record.url?scp=85051128765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051128765&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2018.06.013
DO - 10.1016/j.nano.2018.06.013
M3 - Article
C2 - 30016718
AN - SCOPUS:85051128765
VL - 14
SP - 2191
EP - 2203
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 7
ER -