Conformational changes upon binding of a receptor loop to lipid structures: possible role in signal transduction

Thelma A. Pertinhez; Clóvis R. Nakaie; Regina S H Carvalho; Antonio C M Paiva; Marcel Tabak; Flavio Toma; Shirley Schreier

doi:10.1016/0014-5793(95)01222-Z

Conformational changes upon binding of a receptor loop to lipid structures: possible role in signal transduction

Thelma A. Pertinhez, Clóvis R. Nakaie, Regina S H Carvalho, Antonio C M Paiva, Marcel Tabak, Flavio Toma, Shirley Schreier

Arcispedale Santa Maria Nuova

Research output: Contribution to journal › Article › peer-review

Abstract

The mas oncogene codes for a seven transmembrane helix protein. The amino acid sequence 253-266, from the third extracellular loop and beginning of helix 7, was synthesized either blocked or carrying an amino acid spin label at the N-terminus. Peptide binding to bilayers and micelles was monitored by ESR, fluorescence and circular dichroism. Binding induced tighter lipid packing, and caused an increase of peptide secondary structure. While binding to bilayers occurred only when peptide and phospholipid bore opposite charges, in micelles the interaction took place irrespective of charge. The results suggest that changes in lipid packing could modulate conformational changes in receptor loops related to the triggering of signal transduction.

Original language	English
Pages (from-to)	239-242
Number of pages	4
Journal	FEBS Letters
Volume	375
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01222-Z
Publication status	Published - Nov 20 1995

Keywords

Loop
Peptide-lipid interaction
Receptor
Signal transduction
Spectroscopic technique

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

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10.1016/0014-5793(95)01222-Z

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Conformational changes upon binding of a receptor loop to lipid structures : possible role in signal transduction. / Pertinhez, Thelma A.; Nakaie, Clóvis R.; Carvalho, Regina S H; Paiva, Antonio C M; Tabak, Marcel; Toma, Flavio; Schreier, Shirley.

In: FEBS Letters, Vol. 375, No. 3, 20.11.1995, p. 239-242.

Research output: Contribution to journal › Article › peer-review

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