Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes

E. Rossi, L. Della Casa, S. Piana, A. Iannone

Research output: Contribution to journalArticle

Abstract

Conjugated linoleic acid (CLA) is a polyunsaturated fatty acid, which has been recently proven to be effective in reducing body fat mass, but brings as a side effect, the liver enlargement due to an increased lipid content. The in vivo lipogenic activity has been suggested to be due to the reduction in fat mass and to the consequent metabolism of blood glucose to fatty acid in the liver rather than in the adipose tissue. We investigated the ability of CLA to directly induce steatosis by modulating the expression pattern of hepatic proteins involved in lipid metabolism. To avoid interferences derived from CLA metabolism by other tissues, we used the in vitro model of freshly isolated rat hepatocytes incubated in the presence of different CLA isomers. The direct effect of CLA on lipid accumulation in hepatocytes was demonstrated by the altered expression pattern of several proteins involved in lipid metabolism, as assessed by two-dimensional gel electrophoresis and confirmed by Western blotting analysis. The CLA isomer c9,t11 was most effective in modulating the protein expression profile.

Original languageEnglish
Pages (from-to)511-527
Number of pages17
JournalGenes and Nutrition
Volume7
Issue number4
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Conjugated Linoleic Acids
Hepatocytes
Proteins
Lipid Metabolism
Adipose Tissue
Liver
Lipids
Electrophoresis, Gel, Two-Dimensional
Unsaturated Fatty Acids
Blood Glucose
Fatty Acids
Western Blotting
Fats

Keywords

  • 2DE
  • CLA
  • Hepatocyte
  • Lipid metabolism
  • Proteomics

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Genetics

Cite this

Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes. / Rossi, E.; Casa, L. Della; Piana, S.; Iannone, A.

In: Genes and Nutrition, Vol. 7, No. 4, 10.2012, p. 511-527.

Research output: Contribution to journalArticle

Rossi, E. ; Casa, L. Della ; Piana, S. ; Iannone, A. / Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes. In: Genes and Nutrition. 2012 ; Vol. 7, No. 4. pp. 511-527.
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