Abstract
The presence of haemolysis in a biological blood sample is mainly a result of haemolytic anaemia or haemolysis in vitro. Another cause may be inappropriate handling at the time of collection and processing of the sample, and this may affect the reliability of the test results. Haemolysis is assessed by quantification of free haemoglobin, whose limit is 20 mg/l in plasma and 50 mg/l in serum. Haemolysis can be observed visually when the concentration of free haemoglobin exceeds 300 mg/l. Since haemolysis is the most frequent reason for a biological sample to be considered unsuitable for processing in the clinical laboratory, with a prevalence approaching 3% of all samples received, these consensus recommendations have been drafted specifically to assist laboratory professionals in the detection and management of haemolysed specimens. The recommended approach can be summarized as follows: (1) systematic detection and quantification of haemolysis by visual inspection and subsequent quantification of the haemolysis index in all samples with visually detectable haemolysis; (2) immediate notification to the referring department of the presence of haemolysis in the sample, as locally determined; (3) suspension of all tests affected by the presence and/or the degree of haemolysis detected; and (4) timely request for a second sample to allow the tests previously suspended to be performed.
| Original language | Italian |
|---|---|
| Pages (from-to) | 144-155 |
| Number of pages | 12 |
| Journal | Rivista Italiana della Medicina di Laboratorio |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2011 |
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ASJC Scopus subject areas
- Biochemistry, medical
- Medical Laboratory Technology
Cite this
Raccomandazioni di consenso SIBioC-SIMeL per la rilevazione e gestione dei campioni emolizzati e utilizzo dell'indice di emolisi. / Lippi, Giuseppe; Caputo, Marco; Banfi, Giuseppe; Daves, Massimo; Dolci, Alberto; Montagnana, Martina; Miconi, Valentino; Milanesi, Bruno; Morandini, Margherita; Piva, Elisa; Salvagno, Gian Luca; Troiano, Teresa; Giavarina, Davide.
In: Rivista Italiana della Medicina di Laboratorio, Vol. 7, No. 3, 09.2011, p. 144-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Raccomandazioni di consenso SIBioC-SIMeL per la rilevazione e gestione dei campioni emolizzati e utilizzo dell'indice di emolisi
AU - Lippi, Giuseppe
AU - Caputo, Marco
AU - Banfi, Giuseppe
AU - Daves, Massimo
AU - Dolci, Alberto
AU - Montagnana, Martina
AU - Miconi, Valentino
AU - Milanesi, Bruno
AU - Morandini, Margherita
AU - Piva, Elisa
AU - Salvagno, Gian Luca
AU - Troiano, Teresa
AU - Giavarina, Davide
PY - 2011/9
Y1 - 2011/9
N2 - The presence of haemolysis in a biological blood sample is mainly a result of haemolytic anaemia or haemolysis in vitro. Another cause may be inappropriate handling at the time of collection and processing of the sample, and this may affect the reliability of the test results. Haemolysis is assessed by quantification of free haemoglobin, whose limit is 20 mg/l in plasma and 50 mg/l in serum. Haemolysis can be observed visually when the concentration of free haemoglobin exceeds 300 mg/l. Since haemolysis is the most frequent reason for a biological sample to be considered unsuitable for processing in the clinical laboratory, with a prevalence approaching 3% of all samples received, these consensus recommendations have been drafted specifically to assist laboratory professionals in the detection and management of haemolysed specimens. The recommended approach can be summarized as follows: (1) systematic detection and quantification of haemolysis by visual inspection and subsequent quantification of the haemolysis index in all samples with visually detectable haemolysis; (2) immediate notification to the referring department of the presence of haemolysis in the sample, as locally determined; (3) suspension of all tests affected by the presence and/or the degree of haemolysis detected; and (4) timely request for a second sample to allow the tests previously suspended to be performed.
AB - The presence of haemolysis in a biological blood sample is mainly a result of haemolytic anaemia or haemolysis in vitro. Another cause may be inappropriate handling at the time of collection and processing of the sample, and this may affect the reliability of the test results. Haemolysis is assessed by quantification of free haemoglobin, whose limit is 20 mg/l in plasma and 50 mg/l in serum. Haemolysis can be observed visually when the concentration of free haemoglobin exceeds 300 mg/l. Since haemolysis is the most frequent reason for a biological sample to be considered unsuitable for processing in the clinical laboratory, with a prevalence approaching 3% of all samples received, these consensus recommendations have been drafted specifically to assist laboratory professionals in the detection and management of haemolysed specimens. The recommended approach can be summarized as follows: (1) systematic detection and quantification of haemolysis by visual inspection and subsequent quantification of the haemolysis index in all samples with visually detectable haemolysis; (2) immediate notification to the referring department of the presence of haemolysis in the sample, as locally determined; (3) suspension of all tests affected by the presence and/or the degree of haemolysis detected; and (4) timely request for a second sample to allow the tests previously suspended to be performed.
KW - Hemolysis
KW - Hemolysis index
KW - Hemolytic anemia
KW - Preanalytical variability
KW - Unsuitable samples
UR - http://www.scopus.com/inward/record.url?scp=80955145209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80955145209&partnerID=8YFLogxK
U2 - 10.1007/s13631-011-0022-z
DO - 10.1007/s13631-011-0022-z
M3 - Articolo
AN - SCOPUS:80955145209
VL - 7
SP - 144
EP - 155
JO - Rivista Italiana della Medicina di Laboratorio
JF - Rivista Italiana della Medicina di Laboratorio
SN - 1825-859X
IS - 3
ER -