Conserved T cell and natural killer cell function in treatment-experienced adults receiving tenofovir plus didanosine as nucleoside reverse transcription inhibitor backbone

P. Costa, F. Bozzano, D. Fenoglio, A. Beltrame, G. Cenderello, A. Di Biagio, G. Ferrea, G. Pagano, A. De Maria

Research output: Contribution to journalArticle

Abstract

Anti-retroviral treatment (ART) usually results in efficient control of virus replication and in immune reconstitution. Among potential adverse effects, impairment of immune responses in terms of CD4+ T cell counts has been attributed to some ART regimens, as with didanosine-tenofovir. We studied the functional integrity of adaptive and innate immunity during didanosine-tenofovir-containing ART. Two groups of extensively pretreated patients completing at least 48 weeks of ART containing either lamivudine-didanosine (n = 21) or tenofovir-didanosine (n = 25) were identified. In addition to standard clinical immune and virological parameters, we performed a flow cytometric analysis of natural killer (NK) cells, of memory and naive CD4+ T cells and of T cell receptor αβ+ T cells co-expressing inhibitory NK receptors. Functional analysis consisted in specific and total interferon-γ production by NK cells and of recall antigen proliferation of peripheral blood mononuclear cells. Comparable clinical immunological reconstitution and virological control were confirmed in the two groups of patients in the absence of clinically relevant adverse effects. The proportion of CD4+CD45RA+ T cells and of functionally inhibited killer immunoglobulin-like receptor T cell receptor αβ+ cells, the proliferation to recall antigens as well as NK cell phenotype and function as determined by interferon-γ production in patients treated with tenofovir-didanosine were comparable to those treated with a different regimen. Thus, no differences in functional innate or adaptive immune reconstitution are detected in drug-experienced human immunodeficiency virus-infected patients on tenofovir-didanosine nucleoside reverse transcription inhibitor regimens.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalClinical and Experimental Immunology
Volume158
Issue number1
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Tenofovir
Didanosine
Nucleosides
Natural Killer Cells
Reverse Transcription
T-Lymphocytes
KIR Receptors
T-Cell Antigen Receptor
Interferons
Therapeutics
Antigens
Lamivudine
Adaptive Immunity
CD4 Lymphocyte Count
Virus Replication
Innate Immunity
Blood Cells
Cell Proliferation
HIV

Keywords

  • Didanosine
  • Immune reconstitution
  • NK cell
  • Proliferation
  • Tenofovir

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Conserved T cell and natural killer cell function in treatment-experienced adults receiving tenofovir plus didanosine as nucleoside reverse transcription inhibitor backbone. / Costa, P.; Bozzano, F.; Fenoglio, D.; Beltrame, A.; Cenderello, G.; Di Biagio, A.; Ferrea, G.; Pagano, G.; De Maria, A.

In: Clinical and Experimental Immunology, Vol. 158, No. 1, 10.2009, p. 55-63.

Research output: Contribution to journalArticle

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