Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens

Cinthia Farina, Dierre Van der Bruggen, Pascale Boël, Giorgio Parmiani, Marialuisa Sensi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study we determined TCR α and β chain nucleotide sequences of HLA-Cw(*)1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the α chain CDR3 for two residues and in the β chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.

Original languageEnglish
Pages (from-to)1463-1466
Number of pages4
JournalInternational Immunology
Volume8
Issue number9
Publication statusPublished - 1996

Fingerprint

Melanoma-Specific Antigens
Cytotoxic T-Lymphocytes
Clone Cells
T-Lymphocytes
Melanoma
Lymphocytes
Epitopes
Nucleotides
Peptides
HLA-C*16 antigen
Genes

Keywords

  • Cytotoxic T lymphocyte
  • Immune recognition
  • Tumor epitopes

ASJC Scopus subject areas

  • Immunology

Cite this

Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens. / Farina, Cinthia; Van der Bruggen, Dierre; Boël, Pascale; Parmiani, Giorgio; Sensi, Marialuisa.

In: International Immunology, Vol. 8, No. 9, 1996, p. 1463-1466.

Research output: Contribution to journalArticle

Farina, Cinthia ; Van der Bruggen, Dierre ; Boël, Pascale ; Parmiani, Giorgio ; Sensi, Marialuisa. / Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens. In: International Immunology. 1996 ; Vol. 8, No. 9. pp. 1463-1466.
@article{b110329de5554314bf27447ef663fef7,
title = "Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens",
abstract = "In this study we determined TCR α and β chain nucleotide sequences of HLA-Cw(*)1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the α chain CDR3 for two residues and in the β chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.",
keywords = "Cytotoxic T lymphocyte, Immune recognition, Tumor epitopes",
author = "Cinthia Farina and {Van der Bruggen}, Dierre and Pascale Bo{\"e}l and Giorgio Parmiani and Marialuisa Sensi",
year = "1996",
language = "English",
volume = "8",
pages = "1463--1466",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens

AU - Farina, Cinthia

AU - Van der Bruggen, Dierre

AU - Boël, Pascale

AU - Parmiani, Giorgio

AU - Sensi, Marialuisa

PY - 1996

Y1 - 1996

N2 - In this study we determined TCR α and β chain nucleotide sequences of HLA-Cw(*)1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the α chain CDR3 for two residues and in the β chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.

AB - In this study we determined TCR α and β chain nucleotide sequences of HLA-Cw(*)1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the α chain CDR3 for two residues and in the β chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.

KW - Cytotoxic T lymphocyte

KW - Immune recognition

KW - Tumor epitopes

UR - http://www.scopus.com/inward/record.url?scp=0029791352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029791352&partnerID=8YFLogxK

M3 - Article

C2 - 8921424

AN - SCOPUS:0029791352

VL - 8

SP - 1463

EP - 1466

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 9

ER -