Conserved TCR usage by HLA-Cw(*)1601-restricted T cell clones recognizing melanoma antigens

Cinthia Farina, Dierre Van der Bruggen, Pascale Boël, Giorgio Parmiani, Marialuisa Sensi

Research output: Contribution to journalArticle

Abstract

In this study we determined TCR α and β chain nucleotide sequences of HLA-Cw(*)1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the α chain CDR3 for two residues and in the β chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.

Original languageEnglish
Pages (from-to)1463-1466
Number of pages4
JournalInternational Immunology
Volume8
Issue number9
Publication statusPublished - 1996

Keywords

  • Cytotoxic T lymphocyte
  • Immune recognition
  • Tumor epitopes

ASJC Scopus subject areas

  • Immunology

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