Constitutive autotaxin transcription by Nmyc-amplified and non-amplified neuroblastoma cells is regulated by a novel AP-1 and SP-mediated mechanism and abrogated by curcumin

Antonietta R. Farina, Lucia Cappabianca, Pierdomenico Ruggeri, Natalia Di Ianni, Marzia Ragone, Stefania Merolle, Kimihiko Sano, Mary L. Stracke, Jonathan M. Horowitz, Alberto Gulino, Andrew R. MacKay

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The motility, angiogenesis and metastasis-stimulating factor Autotaxin (Atx), over expressed by human neuroblastomas (NB), is constitutively expressed by human Nmyc-amplified SK-N-BE and non-Nmyc-amplified SH-SY5Y NB cells. Here, we characterise a novel Atx transcriptional mechanism, utilised by both cell lines, that is restricted to the first 285 bp of the Atx promoter and involves AP-1 and SP transcription factors, acting through a CRE/AP-1-like element at position -142 to -149 and a GAbox at position -227 to -235 relative to the Atx translational start site. This novel transcriptional mechanism can be inhibited by internally initiated SP-3 and the natural phenol curcumin.

Original languageEnglish
Pages (from-to)3681-3691
Number of pages11
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - Oct 19 2012

Fingerprint

Curcumin
Transcription Factor AP-1
Transcription
Neuroblastoma
Phenol
Transcription Factors
Cells
Neoplasm Metastasis
Cell Line

Keywords

  • AP-1
  • Autotaxin
  • Curcumin
  • Internally initiated SP3
  • Neuroblastoma
  • SP
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Constitutive autotaxin transcription by Nmyc-amplified and non-amplified neuroblastoma cells is regulated by a novel AP-1 and SP-mediated mechanism and abrogated by curcumin. / Farina, Antonietta R.; Cappabianca, Lucia; Ruggeri, Pierdomenico; Di Ianni, Natalia; Ragone, Marzia; Merolle, Stefania; Sano, Kimihiko; Stracke, Mary L.; Horowitz, Jonathan M.; Gulino, Alberto; MacKay, Andrew R.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3681-3691.

Research output: Contribution to journalArticle

Farina, AR, Cappabianca, L, Ruggeri, P, Di Ianni, N, Ragone, M, Merolle, S, Sano, K, Stracke, ML, Horowitz, JM, Gulino, A & MacKay, AR 2012, 'Constitutive autotaxin transcription by Nmyc-amplified and non-amplified neuroblastoma cells is regulated by a novel AP-1 and SP-mediated mechanism and abrogated by curcumin', FEBS Letters, vol. 586, no. 20, pp. 3681-3691. https://doi.org/10.1016/j.febslet.2012.08.026
Farina, Antonietta R. ; Cappabianca, Lucia ; Ruggeri, Pierdomenico ; Di Ianni, Natalia ; Ragone, Marzia ; Merolle, Stefania ; Sano, Kimihiko ; Stracke, Mary L. ; Horowitz, Jonathan M. ; Gulino, Alberto ; MacKay, Andrew R. / Constitutive autotaxin transcription by Nmyc-amplified and non-amplified neuroblastoma cells is regulated by a novel AP-1 and SP-mediated mechanism and abrogated by curcumin. In: FEBS Letters. 2012 ; Vol. 586, No. 20. pp. 3681-3691.
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