Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have previously developed HIV-1 Pr55gag-based virus-like particles (HIV-VLPs) as presentation and delivery model using a transient Baculovirus expression system. Here we describe the establishment and characterization of stably transfected insect cell line for the constitutive and reproducible production of HIV-VLPs. The persistence of HIV gag coding gene has been verified in clonal resistant insect cells and the protein expression has been confirmed by Western blot analysis. The resulting HIV-VLPs have been evaluated by standard transmission electron microscopy and their immunogenicity has been evaluated in vivo. Our results demonstrate that this strategy is highly efficient for constitutive expression of conformational enveloped VLPs which can be employed as presentation and delivery system for pathogen as well as tumor-associated antigens. This represents, to our knowledge, the first example of stably transfected insect cell line for the constitutive production of VLPs.

Original languageEnglish
Pages (from-to)6417-6424
Number of pages8
JournalVaccine
Volume28
Issue number39
DOIs
Publication statusPublished - Sep 2010

Fingerprint

virus-like particles
Human immunodeficiency virus 1
Virion
Insects
HIV-1
cell lines
Cell Line
insects
Insect Proteins
gag Genes
Baculoviridae
Neoplasm Antigens
Transmission Electron Microscopy
transmission electron microscopy
Western blotting
protein synthesis
Western Blotting
immune response
HIV
antigens

Keywords

  • Baculovirus expression system
  • Delivery system
  • HIV
  • Virus-like particles

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system. / Tagliamonte, M.; Visciano, M. L.; Tornesello, M. L.; De Stradis, A.; Buonaguro, F. M.; Buonaguro, L.

In: Vaccine, Vol. 28, No. 39, 09.2010, p. 6417-6424.

Research output: Contribution to journalArticle

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