The development of a new method is reported which allows the continuous determination of the oxygen dissociation curve for microsamples (600 μl) of whole blood under conditions of pH, p(co 2), methemoglobin concentration, and 2,3 diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H 2O 2 in the presence of catalase (EC 18.104.22.168), to produce the reaction H 2O 2→H 2O + 1/2 O 2. Because the total oxygen content of blood can be derived from the known rate of H 2O 2 addition and the p(o 2) is determined in the liquid phase by an oxygen electrode, the two functions (total O 2 content) and (% oxygen saturation) vs. p(o 2) are simple to calculate. p(co 2) and pH are controlled by adding the base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.
|Number of pages||7|
|Publication status||Published - 1975|
ASJC Scopus subject areas
- Clinical Biochemistry