Control and recognition of anionic ligands in myoglobin

Francesca Cutruzzolà, Carlo Travaglini Allocatelli, Paolo Ascenzi, Martino Bolognesi, Stephen G. Sligar, Maurizio Brunori

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Equilibrium and kinetic experiments on site-directed mutants of a synthetic sperm whale myoglobin (Mb) gene have been performed. Results on the reactivity on both ferric and ferrous wild type and mutants Mb's are presented. Analysis of ligand binding to His(E7) Val and His(E7) Val-Thr(E10) Arg mutants compared to wild-type sperm whale, horse and Aplysia limaelna Mb's, shows that the introduction of an arginyl residue at the topological position E10 greatly enhances the stability of the various Mb:heme ligand adducts. Alternative mechanisms of ligand stabilization may therefore be operative in Mb's lacking the distal histidine.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalFEBS Letters
Volume282
Issue number2
DOIs
Publication statusPublished - May 6 1991

Fingerprint

Myoglobin
Sperm Whale
Ligands
Aplysia
Heme
Histidine
Horses
Stabilization
Genes
Kinetics
Experiments

Keywords

  • Ligand binding
  • Myoglobin mutant
  • Protein engineering

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cutruzzolà, F., Allocatelli, C. T., Ascenzi, P., Bolognesi, M., Sligar, S. G., & Brunori, M. (1991). Control and recognition of anionic ligands in myoglobin. FEBS Letters, 282(2), 281-284. https://doi.org/10.1016/0014-5793(91)80495-O

Control and recognition of anionic ligands in myoglobin. / Cutruzzolà, Francesca; Allocatelli, Carlo Travaglini; Ascenzi, Paolo; Bolognesi, Martino; Sligar, Stephen G.; Brunori, Maurizio.

In: FEBS Letters, Vol. 282, No. 2, 06.05.1991, p. 281-284.

Research output: Contribution to journalArticle

Cutruzzolà, F, Allocatelli, CT, Ascenzi, P, Bolognesi, M, Sligar, SG & Brunori, M 1991, 'Control and recognition of anionic ligands in myoglobin', FEBS Letters, vol. 282, no. 2, pp. 281-284. https://doi.org/10.1016/0014-5793(91)80495-O
Cutruzzolà F, Allocatelli CT, Ascenzi P, Bolognesi M, Sligar SG, Brunori M. Control and recognition of anionic ligands in myoglobin. FEBS Letters. 1991 May 6;282(2):281-284. https://doi.org/10.1016/0014-5793(91)80495-O
Cutruzzolà, Francesca ; Allocatelli, Carlo Travaglini ; Ascenzi, Paolo ; Bolognesi, Martino ; Sligar, Stephen G. ; Brunori, Maurizio. / Control and recognition of anionic ligands in myoglobin. In: FEBS Letters. 1991 ; Vol. 282, No. 2. pp. 281-284.
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