Control of cross-presentation during dendritic cell maturation

Beatriz C. Gil-Torregrosa, Ana Maria Lennon-Duménil, Benedikt Kessler, Pierre Guermonprez, Hidde L. Ploegh, Doriana Fruci, Peter Van Endert, Sebastian Amigorena

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The initiation of most cytotoxic immune responses requires MHC class I-restricted presentation of internalized antigens to CD8+ T lymphocytes, a process called cross-presentation. In dendritic cells (DC), the only antigen-presenting cells that activate naive T cells, cross-presentation is particularly efficient after internalization of opsonized antigens or immune complexes, which are cross-presented through a proteasome- and transporter associated with antigen processing (TAP)-dependent MHC class I antigen presentation pathway. We now show that FcγR-mediated cross-presentation is tightly regulated during DC maturation. Cross-presentation increases soon after activation by lipopolysaccharides, and it is then inhibited in fully mature cells. The initial induction of cross-presentation results from an increase of both antigen internalization and delivery to the cytosol, and from a slight rise in the activity of the proteasome and TAP. The subsequent block of cross-presentation in mature DC is a consequence of the selective down-modulation of antigen internalization and cytosolic delivery, while proteasome and TAP activities continue to rise. Therefore, FcγR-mediated cross-presentation is regulated during DC maturation by the selective control of antigen internalization and transport to the cytosol.

Original languageEnglish
Pages (from-to)398-407
Number of pages10
JournalEuropean Journal of Immunology
Volume34
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Cross-Priming
Dendritic Cells
Proteasome Endopeptidase Complex
Antigens
Antigen Presentation
Cytosol
CD8 Antigens
T-Lymphocytes
Histocompatibility Antigens Class I
Antigen-Presenting Cells
Antigen-Antibody Complex
Lipopolysaccharides

Keywords

  • Cross-presentation
  • Dendritic cells
  • MHC class I
  • Proteasome
  • TAP

ASJC Scopus subject areas

  • Immunology

Cite this

Gil-Torregrosa, B. C., Lennon-Duménil, A. M., Kessler, B., Guermonprez, P., Ploegh, H. L., Fruci, D., ... Amigorena, S. (2004). Control of cross-presentation during dendritic cell maturation. European Journal of Immunology, 34(2), 398-407. https://doi.org/10.1002/eji.200324508

Control of cross-presentation during dendritic cell maturation. / Gil-Torregrosa, Beatriz C.; Lennon-Duménil, Ana Maria; Kessler, Benedikt; Guermonprez, Pierre; Ploegh, Hidde L.; Fruci, Doriana; Van Endert, Peter; Amigorena, Sebastian.

In: European Journal of Immunology, Vol. 34, No. 2, 02.2004, p. 398-407.

Research output: Contribution to journalArticle

Gil-Torregrosa, BC, Lennon-Duménil, AM, Kessler, B, Guermonprez, P, Ploegh, HL, Fruci, D, Van Endert, P & Amigorena, S 2004, 'Control of cross-presentation during dendritic cell maturation', European Journal of Immunology, vol. 34, no. 2, pp. 398-407. https://doi.org/10.1002/eji.200324508
Gil-Torregrosa BC, Lennon-Duménil AM, Kessler B, Guermonprez P, Ploegh HL, Fruci D et al. Control of cross-presentation during dendritic cell maturation. European Journal of Immunology. 2004 Feb;34(2):398-407. https://doi.org/10.1002/eji.200324508
Gil-Torregrosa, Beatriz C. ; Lennon-Duménil, Ana Maria ; Kessler, Benedikt ; Guermonprez, Pierre ; Ploegh, Hidde L. ; Fruci, Doriana ; Van Endert, Peter ; Amigorena, Sebastian. / Control of cross-presentation during dendritic cell maturation. In: European Journal of Immunology. 2004 ; Vol. 34, No. 2. pp. 398-407.
@article{1f85288cf40245a28b3c7a33677e7a39,
title = "Control of cross-presentation during dendritic cell maturation",
abstract = "The initiation of most cytotoxic immune responses requires MHC class I-restricted presentation of internalized antigens to CD8+ T lymphocytes, a process called cross-presentation. In dendritic cells (DC), the only antigen-presenting cells that activate naive T cells, cross-presentation is particularly efficient after internalization of opsonized antigens or immune complexes, which are cross-presented through a proteasome- and transporter associated with antigen processing (TAP)-dependent MHC class I antigen presentation pathway. We now show that FcγR-mediated cross-presentation is tightly regulated during DC maturation. Cross-presentation increases soon after activation by lipopolysaccharides, and it is then inhibited in fully mature cells. The initial induction of cross-presentation results from an increase of both antigen internalization and delivery to the cytosol, and from a slight rise in the activity of the proteasome and TAP. The subsequent block of cross-presentation in mature DC is a consequence of the selective down-modulation of antigen internalization and cytosolic delivery, while proteasome and TAP activities continue to rise. Therefore, FcγR-mediated cross-presentation is regulated during DC maturation by the selective control of antigen internalization and transport to the cytosol.",
keywords = "Cross-presentation, Dendritic cells, MHC class I, Proteasome, TAP",
author = "Gil-Torregrosa, {Beatriz C.} and Lennon-Dum{\'e}nil, {Ana Maria} and Benedikt Kessler and Pierre Guermonprez and Ploegh, {Hidde L.} and Doriana Fruci and {Van Endert}, Peter and Sebastian Amigorena",
year = "2004",
month = "2",
doi = "10.1002/eji.200324508",
language = "English",
volume = "34",
pages = "398--407",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "wiley",
number = "2",

}

TY - JOUR

T1 - Control of cross-presentation during dendritic cell maturation

AU - Gil-Torregrosa, Beatriz C.

AU - Lennon-Duménil, Ana Maria

AU - Kessler, Benedikt

AU - Guermonprez, Pierre

AU - Ploegh, Hidde L.

AU - Fruci, Doriana

AU - Van Endert, Peter

AU - Amigorena, Sebastian

PY - 2004/2

Y1 - 2004/2

N2 - The initiation of most cytotoxic immune responses requires MHC class I-restricted presentation of internalized antigens to CD8+ T lymphocytes, a process called cross-presentation. In dendritic cells (DC), the only antigen-presenting cells that activate naive T cells, cross-presentation is particularly efficient after internalization of opsonized antigens or immune complexes, which are cross-presented through a proteasome- and transporter associated with antigen processing (TAP)-dependent MHC class I antigen presentation pathway. We now show that FcγR-mediated cross-presentation is tightly regulated during DC maturation. Cross-presentation increases soon after activation by lipopolysaccharides, and it is then inhibited in fully mature cells. The initial induction of cross-presentation results from an increase of both antigen internalization and delivery to the cytosol, and from a slight rise in the activity of the proteasome and TAP. The subsequent block of cross-presentation in mature DC is a consequence of the selective down-modulation of antigen internalization and cytosolic delivery, while proteasome and TAP activities continue to rise. Therefore, FcγR-mediated cross-presentation is regulated during DC maturation by the selective control of antigen internalization and transport to the cytosol.

AB - The initiation of most cytotoxic immune responses requires MHC class I-restricted presentation of internalized antigens to CD8+ T lymphocytes, a process called cross-presentation. In dendritic cells (DC), the only antigen-presenting cells that activate naive T cells, cross-presentation is particularly efficient after internalization of opsonized antigens or immune complexes, which are cross-presented through a proteasome- and transporter associated with antigen processing (TAP)-dependent MHC class I antigen presentation pathway. We now show that FcγR-mediated cross-presentation is tightly regulated during DC maturation. Cross-presentation increases soon after activation by lipopolysaccharides, and it is then inhibited in fully mature cells. The initial induction of cross-presentation results from an increase of both antigen internalization and delivery to the cytosol, and from a slight rise in the activity of the proteasome and TAP. The subsequent block of cross-presentation in mature DC is a consequence of the selective down-modulation of antigen internalization and cytosolic delivery, while proteasome and TAP activities continue to rise. Therefore, FcγR-mediated cross-presentation is regulated during DC maturation by the selective control of antigen internalization and transport to the cytosol.

KW - Cross-presentation

KW - Dendritic cells

KW - MHC class I

KW - Proteasome

KW - TAP

UR - http://www.scopus.com/inward/record.url?scp=1642353588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642353588&partnerID=8YFLogxK

U2 - 10.1002/eji.200324508

DO - 10.1002/eji.200324508

M3 - Article

C2 - 14768044

AN - SCOPUS:1642353588

VL - 34

SP - 398

EP - 407

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -