TY - JOUR
T1 - Control of expression of PLCβ1 by LAC repressor system
T2 - Relationship between nuclear PLCβ1 overexpression and growth factor stimulation
AU - Billi, Anna Maria
AU - Matteucci, Alessandro
AU - Faenza, Irene
AU - Manzoli, Lucia
AU - Rubbini, Silvia
AU - Gilmour, Stewart S R
AU - Rhee, Sue Goo
AU - Cocco, Lucio
PY - 1997/12/8
Y1 - 1997/12/8
N2 - Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β1 (PLC β1) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC β1.
AB - Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β1 (PLC β1) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC β1.
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U2 - 10.1006/bbrc.1997.7778
DO - 10.1006/bbrc.1997.7778
M3 - Article
C2 - 9405244
AN - SCOPUS:0031560253
VL - 241
SP - 122
EP - 126
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -