Control of expression of PLCβ1 by LAC repressor system: Relationship between nuclear PLCβ1 overexpression and growth factor stimulation

Anna Maria Billi; Alessandro Matteucci; Irene Faenza; Lucia Manzoli; Silvia Rubbini; Stewart S R Gilmour; Sue Goo Rhee; Lucio Cocco

doi:10.1006/bbrc.1997.7778

Control of expression of PLCβ1 by LAC repressor system: Relationship between nuclear PLCβ1 overexpression and growth factor stimulation

Anna Maria Billi, Alessandro Matteucci, Irene Faenza, Lucia Manzoli, Silvia Rubbini, Stewart S R Gilmour, Sue Goo Rhee, Lucio Cocco

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article

Abstract

Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β₁ (PLC β₁) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC β₁.

Original language	English
Pages (from-to)	122-126
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	241
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.7778
Publication status	Published - Dec 8 1997

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Billi, A. M., Matteucci, A., Faenza, I., Manzoli, L., Rubbini, S., Gilmour, S. S. R., Rhee, S. G., & Cocco, L. (1997). Control of expression of PLCβ1 by LAC repressor system: Relationship between nuclear PLCβ1 overexpression and growth factor stimulation. Biochemical and Biophysical Research Communications, 241(1), 122-126. https://doi.org/10.1006/bbrc.1997.7778

Control of expression of PLCβ1 by LAC repressor system : Relationship between nuclear PLCβ1 overexpression and growth factor stimulation. / Billi, Anna Maria; Matteucci, Alessandro; Faenza, Irene; Manzoli, Lucia; Rubbini, Silvia; Gilmour, Stewart S R; Rhee, Sue Goo; Cocco, Lucio.

In: Biochemical and Biophysical Research Communications, Vol. 241, No. 1, 08.12.1997, p. 122-126.

Research output: Contribution to journal › Article

Billi, Anna Maria ; Matteucci, Alessandro ; Faenza, Irene ; Manzoli, Lucia ; Rubbini, Silvia ; Gilmour, Stewart S R ; Rhee, Sue Goo ; Cocco, Lucio. / Control of expression of PLCβ1 by LAC repressor system : Relationship between nuclear PLCβ1 overexpression and growth factor stimulation. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 241, No. 1. pp. 122-126.

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