Control of expression of PLCβ1 by LAC repressor system: Relationship between nuclear PLCβ1 overexpression and growth factor stimulation

Anna Maria Billi, Alessandro Matteucci, Irene Faenza, Lucia Manzoli, Silvia Rubbini, Stewart S R Gilmour, Sue Goo Rhee, Lucio Cocco

Research output: Contribution to journalArticle

Abstract

Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β1 (PLC β1) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC β1.

Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume241
Issue number1
DOIs
Publication statusPublished - Dec 8 1997

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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