Control of H-2 expression in transformed nonhaemopoietic cells by autocrine interferon

P. Nanni, L. Landuzzi, G. Nicoletti, C. De Giovanni, M. Giovarelli, E. Lalli, A. Facchini, P. L. Lollini

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The relationship between autocrine interferon (IFN) production and the expression of class I Major Histocompatibility Complex (MHC) membrane glycoproteins in vitro was investigated in a panel of murine transformed cells of nonhaemopoietic origin. The panel included 11 cell lines of H-2Kb haplotype derived from fibrosarcomas, carcinomas and melanoma, and from transformed fibroblasts. IFN activity was detected in the conditioned medium of nine cell lines; fibrosarcomas were among the high IFN producers, while the non-producers were a melanoma clone and a lung carcinoma cell line. A significant correlation was found between IFN production and the expression of H-2K/D glycoproteins, thus suggesting that long-term maintainment of MHC glycoprotein expression in Vitro could be mediated by self produced IFN. Two IFN producer cell lines, MN/MCA1 and R80/17, were cultured in the presence of a blocking antiserum against IFN-α/β: a significant decrease in H-2b expression was observed, thus indicating the existence of an autocrine IFN circuit. Taken together these findings suggest that release of IFN is a frequent event among transformed nonhaemopoietic cells, and that self-produced IFN contributes to the regulation of MHC antigen levels in solid tumours.

Original languageEnglish
Pages (from-to)479-482
Number of pages4
JournalBritish Journal of Cancer
Issue number3
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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